Monday, September 27, 2010 2:00 PM - American Society for ...

61-PANTI-HLA AND ANTI-MICA SPECIFIC ANTIBODIES DEVELOPED DURING THE FOLLOW-UP THREE YEARS CAN PREDICTIVE OF ACUTE REJECTION AND RENAL GRAFTFUNCTION.Jun He, Jian Quan Hou, Xiao Ni Yuan. Jiangsu Institute of Hematology, The First Affiliated Hospital ofSoochow University, Suzhou, Jiangsu, China.Aim: The purpose of this study was to detect dynamic for HLA and MICA could predict development ofacute rejection (AR) and kidney allograft function.Methods: 41 kidney transplant patients were tested for anti -HLA Abs and anti-MICA Abs. 37 patientswere screened using single-antigen beads to determine the HLA and MICA-specific antibody levels at 0,30, 90, 180, 360, 720 and 1080 days of posttransplantation. The patients and donors of HLA and MICAallele typing were determined by PCR-SSOP and DSA and NDSA.Results: 9 patients(21.95%) had pre-existing antibodies in 41 transplantation patients.among them 6patients had anti-MICA antibodies, 2 patients had anti-HLA antibodies, one patient had anti-MICAantibodies and anti-HLA antibodies.Patients with pre-existing anti-HLA Abs had higher acute rejection(66.7%) than patients with pre-existing anti-MICA Abs (33.3%)and patients without Abs (27.3%, (P