CD4:CD8 T-Cell Ratio Differs Significantly in Diffuse Large B-Cell ...

INTERNATIONAL TRENDS IN IMMUNITY VOL.1 NO.2 APRIL 2013ISSN 2326-3121 (Print) ISSN 2326-313X (Online) http://www.researchpub.org/journal/iti/iti.htmlIV. CONCLUSIONAltogether, these data suggest an increased number ofcytotoxic T-lymphocytes, associated to a decreased number ofCD4 + T-cells. Since this inverted median ratio is characteristicof DLBCL, and differs significantly from low grade lymphomasubtypes, the T-cell subset modulations may be involved in thelymphomagenesis processes of DLBCL.ACKNOWLEDGMENTThe authors thank the facility core of cellular imaging (CICC,Centre de Recherche des Cordeliers, Paris F-75006, France),the cytometry facility from Rennes Hospital and Sylvie Baudetfor technical assistance. We are grateful to Jo Ann Cahn for hercareful reading of the manuscript.REFERENCES[1] S. S. Dave, G. Wright, B. Tan, A. Rosenwald, R. D. Gascoyne, W. C.Chan, R. I. Fisher, R. M. Braziel, L. M. Rimsza, T. M. Grogan, T. P.Miller, M. LeBlanc, T. C. Greiner, D. D. Weisenburger, J. C. Lynch, J.Vose, J. O. Armitage, E. B. Smeland, S. Kvaloy, H. Holte, J. Delabie, J. M.Connors, P. M. Lansdorp, Q. Ouyang, T. A. Lister, A. J. Davies, A. J.Norton, H. K. Muller-Hermelink, G. Ott, E. Campo, E. Montserrat, W. H.Wilson, E. S. Jaffe, R. Simon, L. Yang, J. Powell, H. Zhao, N.Goldschmidt, M. Chiorazzi, L. M. Staudt, “Prediction of survival infollicular lymphoma based on molecular features of tumor-infiltratingimmune cells,” N. Engl. J. Med., vol. 351, pp. 2159-2169, Nov. 2004.[2] F. Pagès, A. Berger, M. Camus, F. Sanchez-Cabo, A. Costes, R. Molidor,B. Mlecnik, A. Kirilovsky, M. Nilsson, D. Damotte, T. Meatchi, P.Bruneval, P. H. Cugnenc, Z. Trajanoski, W. H. Fridman, J. Galon,“Effector memory T cells, early metastasis, and survival in colorectalcancer.” N. Engl. J. Med., vol. 353, pp. 2654-2666, Dec. 2005.[3] T. Alvaro, M. Lejeune, M. T. Salvadó, C. Lopez, J. Jaén, R. Bosch, L. E.Pons, “Immunohistochemical patterns of reactive microenvironment areassociated with clinicobiologic behavior in follicular lymphomapatients.” J. Clin. Oncol., vol. 24, pp. 5350-5357, Dec. 2006.[4] D. M. Knowles, J. P. Halper, F. A. Jakobiec, “T-lymphocytesubpopulations in B-cell-derived non-Hodgkin's lymphomas andHodgkin's disease.” Cancer, vol. 54, pp. 644-651, Aug. 1984.[5] W. Gorczyca, J. Weisberger, Z. Liu, P. Tsang, M. Hossein, C. D. Wu, H.Dong, J. Y. Wong, S. Tugulea, S. Dee, M. R. Melamed, Z. Darzynkiewicz,“An approach to diagnosis of T-cell lymphoproliferative disorders byflow cytometry.” Cytometry, vol. 50, pp. 177-190, Jun. 2002.[6] O. Hernandez, T. Oweity, S. Ibrahim, “Is an increase in CD4/CD8 T-cellratio in lymph node fine needle aspiration helpful for diagnosing Hodgkinlymphoma? A study of 85 lymph node FNAs with increased CD4/CD8ratio.” Cytojournal, vol. 2, p. 14, Sep. 2005.[7] S. D. Hudnall, E. Betancourt, E. Barnhart, J. Patel, “Comparative flowimmunophenotypic features of the inflammatory infiltrates of Hodgkinlymphoma and lymphoid hyperplasia.” Cytometry B Clin. Cytom., vol. 74,pp. 1-8, Jan. 2008.[8] J. Muris, C. Meijer, S. Cillessen, W. Vos, J. Kummer, B. Bladergroen, M.Bogman, M. Mackenzie, N. Jiwa, L. Siegenbeek Van Heukelom,G.Ossenkoppele, J. Oudejans, “Prognostic significance of activatedcytotoxic T-lymphocytes in primary nodal diffuse large B-celllymphomas.” Leukemia, vol. 18, pp. 589-596, Mar. 2004.[9] Y. Xu, S. H. Kroft, R. W. McKenna, D. B. Aquino, “Prognosticsignificance of tumour-infiltrating T lymphocytes and T-cell subsets in denovo diffuse large B-cell lymphoma: a multiparameter flow cytometrystudy.” Br. J. Haematol., vol. 112, pp. 945-949, Mar. 2001.48