RNA SPLICING AND PROCESSING

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Intron definition describes the process when a pair of splicing sites arerecognized by interactions involving only the 5′ site and the branchpoint/3′ site.Exon definition describes the process when a pair of splicing sites arerecognized by interactions involving the 5′ site of the intron and also the 5′ of thenext intron downstream.Figure 24.12 There may be multiple routes for initial recognition of 5’and 3’ splice sites.B-16
Following formation of the E complex, theother snRNPs and factors involved insplicing associate with the complex in adefined order.The B1 complex is formed when a trimercontaining the U5 and U4/U6 snRNPsbinds to the A complex containing U1 andU2 snRNPs. This complex is regarded as aspliceosome, since it contains thecomponents needed for the splicingreaction. It is converted to the B2 complexafter U1 is released. The dissociation of U1is necessary to allow other components tocome into juxtaposition with the 5′ splicesite, most notably U6 sn<strong>RNA</strong>. At this pointU5 sn<strong>RNA</strong> changes its position; initially it isclose to exon sequences at the 5′ splicesite, but it shifts to the vicinity of the intronsequencesFigure 24.13 The splicing reactionproceeds through discrete stages inwhich spliceosome formation involvesthe interaction of components thatrecognize the consensus sequences.B-17
Page 1 and 2: RNA SPLICING AND PROCESSINGPre-mRNA
Page 3 and 4: The physical form of hnRNA is hnRNP
Page 5 and 6: Nuclear splice junctions are short
Page 7 and 8: What ensures that the correct pairs
Page 9 and 10: The branch site in yeast ishighly c
Page 11 and 12: The five snRNPs involved in splicin
Page 13 and 14: Binding of U1 snRNP to the 5′ spl
Page 15: The direct way of forming an E comp
Page 19 and 20: •Binding of U5 and U4/U6 snRNPs c
Page 21 and 22: Figure 24.16 The EJC (exon junction
Page 23 and 24: Figure 24.18 Three classes of splic
Page 25 and 26: Figure 24.20 Nuclear splicing and g
Page 27 and 28: The ratio of X chromosomes toautoso
Page 29 and 30: SL RNA is a small RNA that donates
Page 31 and 32: Figure 24.26 The intron in yeast tR
Page 33 and 34: Figure 24.28 The 3’ and 5’ clea
Page 35 and 36: Figure 24.30 Splicing of tRNA requi
Page 37 and 38: 45S RNA is a precursor that contain
Page 39 and 40: RNA polymerase I terminates transcr
Page 41 and 42: •The sequence AAUAAA is a signal
Page 43 and 44: Figure 24.35 The 3 processing compl
Page 45 and 46: •Histone mRNAs are not polyadenyl
Page 47 and 48: Figure 24.38 The rrn operons in E.
Page 49 and 50: Figure 24.40 Uridine is converted t
Page 51: B-51

RNA SPLICING AND PROCESSING

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