RNA SPLICING AND PROCESSING

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Specific exons may be excluded or included inthe RNA product by using or failing to use a pairof splicing junctions.Exons may be extended by changing one of thesplice junctions to use an alternative junction.Sex determination in Drosophila involves a seriesof alternative splicing events in genes coding forsuccessive products of a pathway.P elements of Drosophila show germline-specificalternative splicing (producing a repressor (66 kdor transposase protein, as discussed in chapter16).Figure 24.23 Alternative splicing events that involve both sites maycause exons to be added or substituted.B-28
SL RNA is a small RNA that donates an exon in the trans-splicingreaction of trypanosomes and nematodes.•Splicing reactions usually occur only in cis between splice junctions on thesame molecule of RNA.•trans-splicing occurs in trypanosomes and worms where a short sequence(SL RNA) is spliced to the 5 ends of many precursor mRNAs.•SL RNA has a structure resembling the Sm-binding site of U snRNAs andmay play an analogous role in the reaction.Figure 24.24 Splicingusually occurs only in cisbetween exons carried on thesame physical RNAmolecule, but trans splicingcan occur when specialconstructs are made thatsupport base pairing betweenintrons.1 2 1 4B-29
Page 1 and 2: RNA SPLICING AND PROCESSINGPre-mRNA
Page 3 and 4: The physical form of hnRNA is hnRNP
Page 5 and 6: Nuclear splice junctions are short
Page 7 and 8: What ensures that the correct pairs
Page 9 and 10: The branch site in yeast ishighly c
Page 11 and 12: The five snRNPs involved in splicin
Page 13 and 14: Binding of U1 snRNP to the 5′ spl
Page 15 and 16: The direct way of forming an E comp
Page 17 and 18: Following formation of the E comple
Page 19 and 20: •Binding of U5 and U4/U6 snRNPs c
Page 21 and 22: Figure 24.16 The EJC (exon junction
Page 23 and 24: Figure 24.18 Three classes of splic
Page 25 and 26: Figure 24.20 Nuclear splicing and g
Page 27: The ratio of X chromosomes toautoso
Page 31 and 32: Figure 24.26 The intron in yeast tR
Page 33 and 34: Figure 24.28 The 3’ and 5’ clea
Page 35 and 36: Figure 24.30 Splicing of tRNA requi
Page 37 and 38: 45S RNA is a precursor that contain
Page 39 and 40: RNA polymerase I terminates transcr
Page 41 and 42: •The sequence AAUAAA is a signal
Page 43 and 44: Figure 24.35 The 3 processing compl
Page 45 and 46: •Histone mRNAs are not polyadenyl
Page 47 and 48: Figure 24.38 The rrn operons in E.
Page 49 and 50: Figure 24.40 Uridine is converted t
Page 51: B-51

RNA SPLICING AND PROCESSING

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