RNA SPLICING AND PROCESSING

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Figure 24.21 Alternative forms of splicing may generate a variety ofprotein products from an individual gene. Changing the splice sites mayintroduce termination codons (shown by asterisks) or change readingframes.The large T/ small t antigens of SV40 and theproducts of the adenovirus E1A region are generatedby connecting a varying 5′ site to a constant 3′ site.In T/t antigens - the 5′ site used for T antigenremoves a termination codon that is present in the tantigen m<strong>RNA</strong>, so that T antigen is larger than tantigen.In E1A transcripts, one of the 5′ sites connects to thelast exon in a different reading frame, again makinga significant change in the C-terminal part of theprotein.B-26
The ratio of X chromosomes toautosomes determines theexpression of sxl, and changes inexpression are passedsequentially through the othergenes to dsx, the last in thepathway.Presence of Sxl protein changesthe splicing of the transformer(tra) geneFigure 24.22 Sex determination in D. melanogaster involves a pathway inwhich different splicing events occur in females. Blocks at any stage of thepathway result in male development.B-27
Page 1 and 2: RNA SPLICING AND PROCESSINGPre-mRNA
Page 3 and 4: The physical form of hnRNA is hnRNP
Page 5 and 6: Nuclear splice junctions are short
Page 7 and 8: What ensures that the correct pairs
Page 9 and 10: The branch site in yeast ishighly c
Page 11 and 12: The five snRNPs involved in splicin
Page 13 and 14: Binding of U1 snRNP to the 5′ spl
Page 15 and 16: The direct way of forming an E comp
Page 17 and 18: Following formation of the E comple
Page 19 and 20: •Binding of U5 and U4/U6 snRNPs c
Page 21 and 22: Figure 24.16 The EJC (exon junction
Page 23 and 24: Figure 24.18 Three classes of splic
Page 25: Figure 24.20 Nuclear splicing and g
Page 29 and 30: SL RNA is a small RNA that donates
Page 31 and 32: Figure 24.26 The intron in yeast tR
Page 33 and 34: Figure 24.28 The 3’ and 5’ clea
Page 35 and 36: Figure 24.30 Splicing of tRNA requi
Page 37 and 38: 45S RNA is a precursor that contain
Page 39 and 40: RNA polymerase I terminates transcr
Page 41 and 42: •The sequence AAUAAA is a signal
Page 43 and 44: Figure 24.35 The 3 processing compl
Page 45 and 46: •Histone mRNAs are not polyadenyl
Page 47 and 48: Figure 24.38 The rrn operons in E.
Page 49 and 50: Figure 24.40 Uridine is converted t
Page 51: B-51

RNA SPLICING AND PROCESSING

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