MIC - seafdec.org.my

Solvent Extraction• 100 gm dried powdered plant were immersed separately in 500ml of methanol (80 %) & ethanol (90%) for 72 h at roomtemperature• Extract filtered through a Buchner funnel with Whatan No.1 filterpaper• Filtrate was evaporated to dryness with a rotary evaporator at60 °C• Extracts re-suspended in Methanol/Ethanol to achieve the stockconcentration 100 mg/ml• Plants stored in dark vials/bottles at 4 o C until further use for theantimicrobial test.