MIC - seafdec.org.my

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Azadirachta indica• No/weak antibacterial activityin extracts of against all thetested pathogens• Results are contradictory withsome researchers whoreported antibacterial activityof the above herbs (Das et al,1999; Hasna-Banu, 2005 ;Haniffa et al, 2006; Mahfuzul-Hoque et al, 2007,)• Variation due to:– Dose used– Method of extraction– Age– Parts of herbs used
• Crude extracts of all herbs tested havenegligible effect on growth of Pseudomonas sp.(Babayi, 2003)• <strong>MIC</strong> value for V. alginolyticus > V. vulnificus andP. damsela sp for P. betle; higher dosesrequired in infections where V. alginolyticus isthe aetiologic agent• The <strong>MIC</strong> < MBC value of four active plantextracts ; Extracts were bacteriostatic at lowerconcentration & bactericidal at higherconcentration.
Page 1 and 2: MARINE FINFISH PRODUCTION AND RESEA
Page 3 and 4: • Aquaculture is a rapidly expand
Page 5 and 6: • Non-therapeutic alternatives su
Page 7 and 8: • To determine the antibacterial
Page 9 and 10: Azadirachta indica• Local name :S
Page 11 and 12: Terminalia catappa• Local name :
Page 13 and 14: • Vibrio alginolyticus• Vibrio
Page 15 and 16: Preparation of Bacteria• Four dif
Page 17 and 18: PREPARATION OFCRUDE EXTRACT- 2 type
Page 19 and 20: Solvent Extraction• 100 gm dried
Page 21 and 22: ANTIBACTERIAL SUSCEPTIBILITY TESTPa
Page 23: Grading of result (MacKeen et al, 1
Page 26 and 27: METHODS TO DETERMINE MBC• 100µl
Page 28: RESULT
Page 31 and 32: Azadiracta indica
Page 33 and 34: Piper betle
Page 35 and 36: Terminalia catappa
Page 37 and 38: Table 2. Minimum inhibitory concent
Page 39 and 40: MBC• The MIC of the plant extract
Page 41: • MeOH & EtOH extracts of P.betle
Page 45 and 46: • EtOH & MeOH efficient in extrac
Page 47 and 48: • EtOH & MeOH of P.betle and T.ca
Page 49: THANK YOU

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