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Poster PresentationExamining the Conformational Dynamics of Proteins and snRNAsduring Splicing using smFRETWilliams DixonMatthew Kahlscheuer, Dept. of Chemistry, University of MichiganAdvisor: Nils Walter, Dept. of Chemistry, University of MichiganThe spliceosome is a complex cellular machine responsible for catalyzing the two step process inwhich noncoding introns are removed from precursor messenger RNA (pre-mRNA) transcriptsfollowed by ligation of the flanking coding exon sequences to produce mature messenger RNA(mRNA) through a process termed splicing. Spliceosome assembly upon a pre-mRNA substraterequires the stepwise coordination of five small nuclear RNAs (snRNAs) and numerous proteinswith the purpose of creating mRNA that can be correctly transcribed by the ribosome. Given thehighly dynamic nature of the spliceosome and the lack of ideal tools available to study splicing,many specific steps of splicing and spliceosome assembly are not well understood. Singlemoleculefluorescence microscopy tools have recently been developed in the Walter Laboratorythat allow for a more comprehensive study of splicing. In an effort to expand on thesetechniques, this study has two components. The first objective was to investigate whether a vitalheat resistant first step protein, Cwc25, undergoes conformational changes prior to or during thefirst step of chemistry. This required recombinant expression and purification of a modifiedCwc25 protein containing cysteine residues at the N and C-termini for incorporation of FRETpairfluorescent dyes onto the protein. The second objective was to observe the relationshipbetween the U6 and U2 snRNA during catalysis. Endogenous U6 and U2 snRNAs weredepleted from yeast cell extract using DNA oligonucleotides D1 and SRU2 which anneal to U6and U2, respectively, allowing for recognition and digestion by RNaseH. This depleted extractcan then be reconstituted with in vitro transcribed, 3’ labeled (Cy3) U6 snRNA and 5’ labeled(Cy5) U2 snRNA. Using a spliceosome stalled immediately prior to the first step of splicing, therelationship between the U6 and U2 snRNA can be monitored using single-molecule FRET.48
Poster PresentationSynonymous Codon UsageSimone DozierAdvisor: Scott Emrich, Dept. of Computer Science and Engineering, University of Notre DameCodons are nucleotide triplets that code for amino acids. A set of codons that correspond to thesame amino acid are “synonymous”. Surprisingly, which synonym is used can have an effect ontranslation speed and the final structure of the folded protein. I generated visuals for existingprojects, and investigated whether codon functionality extended to viruses.49
Page 1: SUMMER UNDERGRADUATERESEARCH SYMPOS
Page 4 and 5: Oral Presentations ScheduleBiology,
Page 6 and 7: Physics Jordan Room 322Session III1
Page 8 and 9: Heather Fick, Jennifer Parra, Kirin
Page 10 and 11: Nathaniel Wirgau - Synthesis and Ch
Page 12 and 13: Cameron Moore - Optimizing Paper Te
Page 14 and 15: Poster PresentationEspN and FL-EC7
Page 16 and 17: Poster PresentationThermal Rectifie
Page 18 and 19: Poster PresentationSynthesis of a G
Page 20 and 21: Poster PresentationLight It Up: Hig
Page 22 and 23: Poster PresentationExternal modulat
Page 24 and 25: Poster PresentationCreating a Devic
Page 26 and 27: Poster PresentationDevelopment of a
Page 28 and 29: Poster PresentationElectrostatic Do
Page 30 and 31: Poster PresentationBiological Chara
Page 32 and 33: Oral PresentationDamage of DNA in D
Page 34 and 35: Poster PresentationKnockdown of G-p
Page 36 and 37: Poster PresentationInteger Solution
Page 38 and 39: Poster PresentationTcpP Diffusion R
Page 40 and 41: Poster PresentationThe New Digital
Page 42 and 43: Poster PresentationSynthesis of Nic
Page 44 and 45: Poster PresentationModeling the For
Page 46 and 47: Poster PresentationInverse Islands:
Page 50 and 51: Poster PresentationPlasmon-Enhanced
Page 52 and 53: Poster PresentationCharacterization
Page 54 and 55: Poster PresentationSynthesis of α-
Page 56 and 57: Poster PresentationNon-Invasive Opt
Page 58 and 59: Poster PresentationAn Investigation
Page 60 and 61: Poster PresentationVisualizing Mism
Page 62 and 63: Poster PresentationMaking Gasoline
Page 64 and 65: Poster PresentationPrivacyRangerDan
Page 66 and 67: Oral PresentationUnderstanding Stro
Page 68 and 69: Poster PresentationEnzymatic Synthe
Page 70 and 71: Poster PresentationVisualizing Imit
Page 72 and 73: Poster PresentationBiomechanical En
Page 74 and 75: Poster PresentationPolymer-Template
Page 76 and 77: Poster PresentationThe Role of GRP7
Page 78 and 79: Poster PresentationFeasibility of g
Page 80 and 81: Poster PresentationA Simple Method
Page 82 and 83: Oral PresentationSyntheses and SAR
Page 84 and 85: Poster PresentationMicrocontact Pri
Page 86 and 87: Poster PresentationAssay Developmen
Page 88 and 89: Poster PresentationInvestigating In
Page 90 and 91: Poster PresentationThermosensitive
Page 92 and 93: Oral PresentationThe World’s Firs
Page 94 and 95: Poster PresentationManipulation of
Page 96 and 97: Oral and Poster PresentationMathema
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Poster PresentationCharacterization
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Oral PresentationExploring the Grow
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Poster PresentationPossible Student
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Poster PresentationEvaluating the E
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Poster PresentationExamining Interp
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Poster PresentationRaman Spectrosco
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Poster PresentationAn Enantioselect
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Poster PresentationRoles and Intera
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Poster PresentationPerformance Eval
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Poster PresentationMechanisms Gover
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Poster PresentationCarbon Dioxide C
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Oral PresentationA Parametric Model
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Poster PresentationAnalysis of hole
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Poster PresentationSynthesis of New
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Poster PresentationEmploying the En
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Poster PresentationIdentifying the
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Poster PresentationThe Geppetto Pro
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Poster PresentationGeneration and c
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Oral PresentationDouble Folding Ana
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Poster PresentationCrystalline InSb
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Poster PresentationPopulation genet
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Poster PresentationFunctional chara
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Poster PresentationDevelopment of s
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Oral PresentationBeam Monitor Proto
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Poster PresentationRole of cyclins
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Poster PresentationHey! Can Someone
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Oral PresentationDelineation of the
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Poster PresentationData Git: Proven
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Poster PresentationDetermining the
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Poster PresentationEffects of pH on
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Oral PresentationExploring the Coll
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Poster PresentationMD Simulations R
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Poster PresentationAnalysis of luna
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Poster PresentationStasis of Saltwa
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Poster PresentationSynthetic proces
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Poster PresentationHurricane and St
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Poster PresentationReceptor Interac
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Poster PresentationSynthesis and Ch
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Oral PresentationSummary of CMS Min
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Oral PresentationConstructing and T
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INDEX TO ABSTRACTSAcevedo, Ephraim
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