Why OCTAX Laser shot

Laser ShotSystem for ARTBased on the scientificwork of specialists atEPFL and CHUVLausanne the mostadvanced 1.48 μminfrared diode lasershave been developed.Lasers using thistechnology presentlyare the most widelyused systems in theworld with the largestnumber of installationsand treatments.EyeWareImaging SoftwareThe OCTAX Laser Shotis the first digitallycontrolled laser systemincluding OCTAXEyeWare imagingsoftware for digitalimage or video processingalso offeringa large number ofadvanced features.Ref.No. 19310/0148Components• OCTAX Laser Shotmodule• OCTAX EyeWareBASIC• OCTAX Laser Lens• OCTAX Eye USBcamera• ComputerCost for OCTAX systemcompatible computersare not included inOCTAX system pricing.Software specificationComputer hardware: Intel Processor based, 2,8 GHz,Microsoft® Windows 7/Vista/XPLaser targeting: Live video display with crosshairand measurement grid overlayMeasurement: Direct calibration and directinteractive measurementImaging software: Image archival includingmeasurement locationsImage database: Access to image data viadatabase organized per patient and treatmentpolarAIDEAutomatic assessment of oocyte quality and beyondOCTAX polarAIDE is the next generation integrated polarization microscopy system based onOCTAX ICSI Guard technology*.Take advantage of OCTAX´s modular high endtechnology for evaluating your oocytes:OCTAX polarAIDE TM is available as stand-alonesystem, as an upgrade to OCTAX ICSI Guard TMor as an add-on to OCTAX Laser Shot TM systems.All functions are handled by one easy-to-usesoftware: OCTAX EyeWare TM is the uniqueplatform for the control of all OCTAX products:laser, polarization microscopy, image processing,data storage and motorized stage.Main Features• Quantification of the birefringence of the zona pellucida• Automatic scoring of the developmental potential of oocytes in seconds• Orientation-independent live imaging of the meiotic spindle• Simultaneous view of oocyte morphology and spindle• Self-calibrating, no active calibration required• One button instant on/off function for convenient use• Spindle imaging under normal light intensity (bright field)• Full OCTAX Laser Shot TM and Fertilase® compatibility, i.e. one camera, one software and onedatabase for laser control, spindle visualization and image processing• Support of all OCTAX EyeWare TM features (image acquisition and archival, video and time lapsevideo recording, biometric measurements etc.)Laser module specificationLaser: 1.48 μm Infrared Diode Laser class 1MPower in Focus: 90-130 mW +/-5 %(depending on the microscope model)Irradiation time: 0.1-10.0 ms, in 0.1 ms stepsDrilling precision: < 1 μmDrilling reproducibility: < 1 μmDrilling range with one shot: ~ 1 μm to ~ 25 μm(depending on the zona pellucida characteristics)Beam position stability: < 5 μm per monthOCTAX USB 2.0 cameraResolution: 1280 x 1024 pixelFrame rate: up to 30 fpsOCTAX Laser Shot system has received 510(k) clearance by the FDA/USA for Laser Assisted HatchingMetaphase II oocyte, polarAIDE TM in bright fieldoptics.Metaphase II oocyte, polarAIDE TM active, combinedmode using red/green processed image. Red areaindicates high birefringence.Metaphase II oocyte, polarAIDE TM active scoringmode. Pink area indicates high birefringence.Colour-coded numerical score for oocyte qualityis displayed automatically.System Components• Circular polarizer and GIF filter• Liquid crystal analysis slider• High resolution OCTAX USB 2.0 digital camera• OCTAX polarAIDE TM softwarepolarAIDE TM is currently available for• Nikon Ti, TE2000, TE200/300, Diaphot 200/300• Olympus IX 50/70 and IX 51/71• Zeiss Axio Observer, Axiovert 200, 135, 100• Leica DMIRB, DMI 4000, DMI 6000• other microscopes on requestFor operating OCTAX polarAIDE TM , oocytes have to be placed in glass bottom dishes instead of plastic dishes. A heated stage is mandatory to maintain optimumtemperature. OCTAX polarAIDE TM supports high resolution live video display (1280x1024 pixel) on selected computer hardware.* Patents pending. polarAIDE TM is currently not available in U.S.A.Photo courtesy CHUV, Lausanne, Switzerland20 21

cytoScreenAffordable IMSI through digital image enhancementVibration Free Microscopic Table• Compatible with your existing Hoffman ICSI microscope• Computer-enhanced high-magnification microscopy for morphology based sperm selection• No oil immersion, no DIC – practicable for your existing workflow• Cost effective integration into your existing OCTAX environment (camera, EyeWare imaging software)• Total magnification adjustable, up to 7,200 x• Use your existing OCTAX laser for convenient sperm immobilizationI. System componentsCondenserObjectiveCameraSoftwareExisting Hoffman´s or relief contrastCost saving solution, condenser supports all potential applications60 xLarge field of view allows sperm immobilization without changingmagnification1.3 or 3.0 MegapixelII. Features and performanceImage database, one button image capture, biometric measurement tools,one button online zoom-in function, adjustable image enhancementOCTAX polarAIDE TM spindle and zona imaging system and OCTAX LaserShot TM microsurgical laser can be integrated into the same softwareenvironmentVibration free table – speciallydeveloped for micromanipulationin IVF labsFour vibration dampers with combinedrubber metal connection and hydraulicbearingSlabMaterial:Dimensions:FrameMaterial:Height:Weight:polished granite120 x 70 x 10 cm(standard),special sizes and wallmounted versions areavailable on requestStainless steel77 - 82 cm, adjustableapprox. 300 kgRef.No. 22047/0000Oil immersionNoEasy handling, no cleaning, works with or without heated glass plateSharpness of the imageTotal magnificationon the screenAnalysis of spermhead shape and vacuolesDishes requiredCompatibilityGood (limited by the physical characteristics of the 60 x objective)improved through digital image processing of the live video> 10,000 x with intermediate magnification 1.6 xFor practical reasons, the optimum range of magnification is between5,000 x and 7,000 xFeasible without restrictionsGlass bottom dishesCan be mounted to most current inverted microscope models used for ICSI22 23

Why OCTAX Laser shot TM systems? Unique features of OCTAX1. Low power laser system of proven safety in clinical applicationOCTAX TM (the advanced version of Fertilase R ) is absolutely unmatched with regard to its scientificbackground and the safety testing for clinical application. There are more than 70 original paperspublished on the use of Fertilase R / OCTAX TM . Almost all scientific papers describing the use of a 1.48µm laser are based on either Fertilase R or OCTAX TM and, as a matter of fact, based on a laser withlow power in focus of 100 to 150 mW.Our system is the only laser on which basic safety studies have been done. All fundamentalsafety studies regarding the use of a 1.48 µm laser in ART are based on Fertilase R laser. Theyinvolved histological as well as genetic and scanning electron microscope (SEM) analyses. OCTAX TMis the digital version of Fertilase R . Without changing the core settings, OCTAX is manufactured anddeveloped by the same scientists as Fertilase R . Hence, the OCTAX TM concept combines latesttechnology with continuity and proven safety.Competitors are using higher laser power (300 – 400 mW) and different techniques. They do notclearly indicate the power in focus which is the only relevant parameter for clinical application,because the power of the laser diode will be modified according to optical conditions, to the type ofplastic dishes, the volume of culture media etc. It is too simple to conclude that basic test results withFertilase R / OCTAX TM technology are valid for other laser systems which have been continuouslyincreasing laser power. Higher power will lead to higher peak temperatures which cannot becompensated for by just reducing pulse length.The only published follow-up study is based on Fertilase R laser technology: Kanyó K., Konc J.(2001) Follow-up study after non-contact laser hatching. IFFS Melbourne, Abstract Book, p. 49 (posterpresentation). Infertility Center of Buda, St. János Hospital, 1. Diösärok, Budapest, Hungary, 1125.There are around 15,000 babies born after application of Fertilase R / OCTAX TM without any adverseeffect being documented. So why take any risk if you could be on the safe side with the wellestablishedmarket leader using a system of proven safety?2. ReliabilityFertilase R / OCTAX TM Laser shot systems are the most used and best known systems worldwide.During the last 10 years, more than 300 systems have been installed worldwide without anyproblems occurring. Many leading IVF centers, researchers and pioneers in IVF are working with oursystem. Just a few examples:• Centro IVI Valencia, Valencia, Spain (Prof. A. Pellicer Martínez)• Department of Gynecological Endocrinology and Reproductive Medicine, University of Bonn,Germany (Dr. M. Montag, Prof. van der Ven; 2 systems)• Free University of Brussels, Belgium (Prof. Van Steirteghem, Prof. Devroy; 2 systems)• Hôpital Antoine Béclère, Clamart, France (Dr. R. Fanchin)• Kato Ladies Clinic, Tokyo, Japan• Obstetrics and Gynecology Hospital, Chongquing, China (Dr. Huang)• Reproductive Biology Associates, Atlanta, USA (Z.P. Nagy, MD, PhD),• Sydney IVF, Sydney, Australia (Dr. J. Catt)• The Egyptian IVF-ET Centre, Cairo, Egypt (Prof. Aboulghar, Prof. Mansour)

OCTAX TM laser shot system is a maintenance free system. There are no parts to be replaced inspecific intervals nor movable parts prone to disturbances. If necessary, cleaning of opticalcomponents can be done by our local distributors as well as by technicians of microscopemanufacturers. System updates will be provided within short response times by technicians from MTGMedical Technology Vertriebs-GmbH, Germany.3. Unmatched image qualityOCTAX Laser Shot TM is using the latest imaging and computer technologies. It is the only laser shotsystem working with digital instead of analogue cameras. The system excels through outstandingimage quality (see our brochures; all images taken with OCTAX USB camera on a Nikon TE2000inverted microscope). Only OCTAX is offering two high quality laser objectives (25 x, 40 x) fittingany demands. The objectives support Hoffman´s Modulation Contrast as well as Zeiss PlasDICfor high quality images and live video.4. Advanced features of the OCTAX “all in one” systemThe OCTAX TM system only needs one USB 2 camera and the EyeWare TM image processing anddatabase software for controlling the laser shot system, archiving images and videos, visualizingmeiotic spindles and even for controlling the OCTAX TM motorized and heated microscope stages.There are no additional controllers needed.OCTAX EyeWare TM is the only system coming with a microsurgical laser which offers in one system• online Web access to patient database, images and video files• unlimited number of images stored in patient database• multi-language software editions: English, German, Chinese, Japanese (additional languages inpreparation)• digital video recording / time lapse video recording without any limit in duration / file size• image capturing from any video source (analogue and digital)• energy guard TM safety system for laser application• the potential to fully integrate the OCTAX polarAIDE TM spindle imaging software at any time5. OCTAX TM has the experience and know-how – through pioneering work‣ first birth after laser assisted hatching (1.48 µm)‣ first birth after laser assisted blastomere biopsy and PGD‣ first birth after laser assisted polar body biopsy‣ first birth after laser assisted immobilization of spermatozoa (an application to avoid the use ofPVP with ICSI)‣ first pregnancy after laser assisted zona drilling for ICSI‣ first ongoing pregnancy after laser assisted removal of necrotic blastomeres from frozen-thawedembryosSummaryIn summary, the OCTAX Laser shot system TM for ART was developed by the leading scientistsin this field, based on the longest experience with microsurgical laser technology. It wasintroduced into clinical routine and tested to guarantee optimal safety in close cooperationwith embryologists all over the world. A network of disciplines complementing each otherenables OCTAX to continuously improve the laser shot system and convert trend-setting ideasinto practical application. Fertilase R and its successor OCTAX laser shot system TM is theoriginal and the gold standard in ART laser technology.TM – Safety – Experience – Precision