Breast Cancer - Arab Medical Association Against Cancer

Prognostic factors 220• Metallothionein isoform3 (MT-3) is a matrix component. Its regulation isaberrant in breast cancer and its overexpression is associated with poorprogression.Chromosomal Changes• Numeric aberration of chromosome 1 and 7 indicate abnormal DNA andaggressive tumor.• LOH 3p14 correlates with aggressive biology.• Mismatched repair gene: inactivation of DNA repair in breast tissue couldlead to tumor pathogenesis.Methodologies• Tissue microarray DNA technology: is a new method, which allowsdetection of mutated, deleted or amplified status of up to 1000 genessimultaneously. This forms a library of individual tumor profiles associatedwith sensitivity and resistance to specific cytotoxic treatment. Individualtumor samples can be tested for optimal regimen on the basis of their DNAfingerprint. It could be performed on tumor sample from paraffin blocks.One gene in question could be correlated with the status of numerous othergenes that could affect its function.• Comparative Genomic Hybridization: the genetic profile of two tissuesamples could allow the study of tumor development progression orregression.• Automated Cellular Imaging System can assist the quantitation ofimmunohistochemistry; it is a sensitive and rapid system with objectivefindings.