Post Transcriptional Gene Silencing in Plants (PTGS) - Eurasnet.info

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POST TRANSCRIPTIONALGENE SILENCING -PTGSPlants: Cosuppression or Epigenetic gene silencing:1990: found that transgenes could suppress the expression of similar endogenousgenes. which resulted in loss of function in 10-40% of the transgenic plants.Exp: Chalcone synthase (anthocyanin synthese pathway ) in PetuniaCaMVGenNOS-TerCaMV: Cauliflower Mosaic Virus PromotorNOS-Ter: Nopalinsynthase TerminatorBoth, endogenous and transgene were transcribed but almost no mRNA wasdetectable, so silencing is on the RNA level RNA silencing (not genesilencing), coding region was methylated.
GENE SILENCINGPTGS: Post transcriptional gene silencingTGS: Transcriptional gene silencingTGSHDGS: Homology dependentgene silencingPTGS-) promotor inactive-) promotor methylated-) chromatin remodelling-) frequently meiotically heritable-)both transgene and endogenousgenes are methylated-) non symmetrical methylation(non CG or CNG) → hallmark ofRNA directed DNA methylation.-) X inactivation: Xist – Tsix:dsRNA? and methylation-) transcribed, but no fulltranscript found-) coding region methylated-) not dependent on ongoingtranslation-) fully reversible during meioses
Page 4 and 5: PTGS can also be induced by homolog
Page 7 and 8: Cross protection: for several virus
Page 9 and 10: Fire, A. TIGS, 15, 358 (1999)
Page 11 and 12: PTGS: siRNAsPlants: 1999: small RNA
Page 13 and 14: An integrated model for RNAi and PT
Page 15 and 16: Systemic silencing: mobile signalsi
Page 17 and 18: Intercellular transport of TMV RNA?
Page 20 and 21: Viruses fight back: suppressors of
Page 22 and 23: RNA-based silencing strategies in p
Page 24 and 25: General pathway of RNAi in vitroRNA
Page 26 and 27: siRNA asymmetry, dsRNA processing a
Page 28: RISC Assembly in Drosophila

Post Transcriptional Gene Silencing in Plants (PTGS) - Eurasnet.info

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