Post Transcriptional Gene Silencing in Plants (PTGS) - Eurasnet.info

More documents

Recommendations

Info

Viruses fight back: suppressors of RNAsilencingtransgenic GFP plantPotato virus Y15 d post InokulationPlant viruses all have RNAsilencing suppressors:PVX-p25 movement protein:affects systemic silencing,HcPro-Potyviruses: suppressproduction of small RNAs,important for virus infectivity
Viral strategies for suppression and evasion of RNA-silencinga | Direct interference with silencing-effectormolecules is illustrated by the tombusviral P19protein. The head-to-tail organization of thetombusviral P19 homodimers (blue and green)allows binding to small interfering RNA (siRNA)duplexes (yellow). Two sets of tryptophanresidues (yellow) bind to the last set of base pairson either end of the siRNA, leading to effectivemeasurement of the duplex length, such that P19selects siRNAs of 21nt for binding. Thesequestered siRNA is prevented from enteringthe RNA induced silencing complex (RISC) andis therefore inactivated.b | Recruitment of endogenous negativeregulators of RNA silencing is illustrated by thepotyviral helper component proteinase (HcPro).HcPro interacts with the calmodulin-like proteinrgsCaM (regulator of gene silencing CaM) toinactivate the RNA-silencing pathway throughan unknown mechanism at an intermediate stepthat involves both RISC and Dicer.
Page 2 and 3: POST TRANSCRIPTIONALGENE SILENCING
Page 4 and 5: PTGS can also be induced by homolog
Page 7 and 8: Cross protection: for several virus
Page 9 and 10: Fire, A. TIGS, 15, 358 (1999)
Page 11 and 12: PTGS: siRNAsPlants: 1999: small RNA
Page 13 and 14: An integrated model for RNAi and PT
Page 15 and 16: Systemic silencing: mobile signalsi
Page 17 and 18: Intercellular transport of TMV RNA?
Page 22 and 23: RNA-based silencing strategies in p
Page 24 and 25: General pathway of RNAi in vitroRNA
Page 26 and 27: siRNA asymmetry, dsRNA processing a
Page 28: RISC Assembly in Drosophila

Post Transcriptional Gene Silencing in Plants (PTGS) - Eurasnet.info

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?