Post Transcriptional Gene Silencing in Plants (PTGS) - Eurasnet.info

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siRNA asymmetry, dsRNA processing and the implicationsfor RISC assembly.a | When 21-nt short interfering (si)RNAs are used to initiate RNA interference (RNAi), the strand with its5‘ terminus at the thermodynamically less stable end of the duplex is preferentially incorporated into theRNA-induced silencing complex (RISC). The guide strand is shown in red and the passenger strand inblue. Less stably base-paired regions of the siRNA are depicted in grey and the more stable regions areshown in black.b | Dicer (Dcr) generates siRNAs from the ends of double-stranded (ds)RNAs. The multiple domains thatare found in most Dcrs (the RNA helicase, DUF283, PAZ, RNase IIIa (RIIIa), RNase IIIb (RIIIb), anddsRNA-binding (dsRBD) domains) are shown. Current models indicate that Dcr interacts with nascentsiRNAs asymmetrically, with its PAZ domain bound to the pre-existing dsRNA end and its dsRBD boundcloser to the dsRNA-cleavage sites (arrows). The dual roles of Dcr in dsRNA cleavage and RISC assemblyindicate that Dcr might remain bound to the siRNA after it is processed from the dsRNA precursor.Combined dsRNA-processing and target-cleavage assays suggest that the strand that has its 3‘ end boundto the PAZ domain preferentially assembles into RISC.
dsRNA processing by dicer and RISC formationpassenger strandguide strandRISC: RNA induced silencing complex.thermodynamic asymmetry rule: for synthetic siRNA strand selection: The strand whichhas the its 5‘ end at the less stably base-paired region becomes the guide strand.
Page 2 and 3: POST TRANSCRIPTIONALGENE SILENCING
Page 4 and 5: PTGS can also be induced by homolog
Page 7 and 8: Cross protection: for several virus
Page 9 and 10: Fire, A. TIGS, 15, 358 (1999)
Page 11 and 12: PTGS: siRNAsPlants: 1999: small RNA
Page 13 and 14: An integrated model for RNAi and PT
Page 15 and 16: Systemic silencing: mobile signalsi
Page 17 and 18: Intercellular transport of TMV RNA?
Page 20 and 21: Viruses fight back: suppressors of
Page 22 and 23: RNA-based silencing strategies in p
Page 24 and 25: General pathway of RNAi in vitroRNA
Page 28: RISC Assembly in Drosophila

Post Transcriptional Gene Silencing in Plants (PTGS) - Eurasnet.info

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