Post Transcriptional Gene Silencing in Plants (PTGS) - Eurasnet.info
Post Transcriptional Gene Silencing in Plants (PTGS) - Eurasnet.info
Post Transcriptional Gene Silencing in Plants (PTGS) - Eurasnet.info
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Chemical Mechanism of RISC-catalysed target-mRNA cleavageSlicer: enzyme which cuts the target strand, strong evidence that Ago prote<strong>in</strong>s possessthis activity. Crystal structure show that the PIWI doma<strong>in</strong> of Ago prote<strong>in</strong>s are very similarto RNaseH and harbour endonuclease activity. Like RNase H it is dependent on divalentmetal ions, produces 3‘OH and 5‘P and only cleaves onestrand of the duplex RNA.
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