RNA Splicing: Removal of Introns from Primary ... - EURASNET

More documents

Recommendations

Info

Pre-mRNA splicing• most eukaryotic protein-coding genes areinterrupted with introns• Intron (intervening sequence-IVS) does not code forprotein• Exon – protein coding sequence• Exons relatively short (1 nt)• Introns can be up to several 1,000 nt• Primary transcripts (pre-mRNAs) up to 100,000 nt
Cis elements required for splicing5‘ssBP3‘ssYeastGUAUGUUACUAACYAGVertebratesESEAGGUAAGUCURAYYYYY NCAG10-15GUESEPlantsESE?AG GUAAGUCURAYUGYAG GUUA-richUA-rich6210070 49 64 95100 4479 99 58 53 4210057ESE?5‘ss – 5‘ splice site (donor site)3‘ss – 3‘ splice site (acceptor site)BP – branch point (A is branch point base)YYYY 10-15 – polypyrimidine trackY – pyrimidineR – purineN – any base
Page 1: Modification and processing ofeukar
Page 5 and 6: Chemistry of pre-mRNA splicingtwo c
Page 7 and 8: Spliceosome- large ribonucleoprotei
Page 9 and 10: Recognition of splice sitesdonor (5
Page 11 and 12: U2 snRNA Base Pairs With IntronBran
Page 13 and 14: Step 4: U6 binds the 5’ splice si
Page 15 and 16: U5, U6 Interactions in Splicing
Page 17 and 18: Spliceosome & ATP -> RNA-RNARearran
Page 19 and 20: Spliceosome cycle
Page 21 and 22: 5`and 3`splice site selectionIntron
Page 23 and 24: Alternative pre-mRNA splicing- Freq
Page 25 and 26: Patterns of alternative exon usage
Page 27 and 28: The Troponin T (muscle protein) pre
Page 29 and 30: SR proteinsRRM RRM SRRRM SRRRMZn SR
Page 31 and 32: Negative and Positive Control of Al
Page 33 and 34: Functional antagonism of SF2/ASF (S
Page 35 and 36: Negative regulation of alternative
Page 37 and 38: The Cascade that DeterminesSex in D
Page 39: Alternative RNA Splicing in Drosoph
Page 42 and 43: Mutations that disrupt splicing•
Page 44 and 45: AT-AC introns IA minor class of nuc
Page 46 and 47: Major U2 spliceosomeSRp34U1-70KGUU1
Page 48 and 49: Self-Splicing Introns• Group I in
Page 50 and 51: Self-Splicing Introns - II
Page 52 and 53:
Organisms With Trans-SplicingTrypan
Page 54 and 55:
Trans-splicing• Splicing does not
Page 56:
Trans splicng of polycistronic pre-
show all

RNA Splicing: Removal of Introns from Primary ... - EURASNET

Create successful ePaper yourself

Delete template?

Save as template?