RNA Splicing: Removal of Introns from Primary ... - EURASNET

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How is alternative splicing achieved?Alternative exons often have suboptimal splice sites and/or lengthSplicing of regulated exons is modulated:1. Proteins – SR proteins and hnRNPs2. cis elements in introns and exons – splicing enhancers and silencersDifferences in the activities and/or amounts of general splicingfactors and/or gene-specific splicing regulators duringdevelopment or in differnt tissues can cause alternative splicing
SR proteinsRRM RRM SRRRM SRRRMZn SR- nuclear phosphoproteins, localised in speckles- phosphorylation status regulates theirsubcellular localisation and protein-proteininteractions- shuttling proteins (h9G8, hSRp20, hSF2/ASF)- constitutive splicing- alternative 5` splice site selection- alternative 3` splice site selectionexon-(in)dependent- found in all eukaryotes except in S. cerevisiae
Page 1 and 2: Modification and processing ofeukar
Page 3 and 4: Cis elements required for splicing5
Page 5 and 6: Chemistry of pre-mRNA splicingtwo c
Page 7 and 8: Spliceosome- large ribonucleoprotei
Page 9 and 10: Recognition of splice sitesdonor (5
Page 11 and 12: U2 snRNA Base Pairs With IntronBran
Page 13 and 14: Step 4: U6 binds the 5’ splice si
Page 15 and 16: U5, U6 Interactions in Splicing
Page 17 and 18: Spliceosome & ATP -> RNA-RNARearran
Page 19 and 20: Spliceosome cycle
Page 21 and 22: 5`and 3`splice site selectionIntron
Page 23 and 24: Alternative pre-mRNA splicing- Freq
Page 25 and 26: Patterns of alternative exon usage
Page 27: The Troponin T (muscle protein) pre
Page 31 and 32: Negative and Positive Control of Al
Page 33 and 34: Functional antagonism of SF2/ASF (S
Page 35 and 36: Negative regulation of alternative
Page 37 and 38: The Cascade that DeterminesSex in D
Page 39: Alternative RNA Splicing in Drosoph
Page 42 and 43: Mutations that disrupt splicing•
Page 44 and 45: AT-AC introns IA minor class of nuc
Page 46 and 47: Major U2 spliceosomeSRp34U1-70KGUU1
Page 48 and 49: Self-Splicing Introns• Group I in
Page 50 and 51: Self-Splicing Introns - II
Page 52 and 53: Organisms With Trans-SplicingTrypan
Page 54 and 55: Trans-splicing• Splicing does not
Page 56: Trans splicng of polycistronic pre-

RNA Splicing: Removal of Introns from Primary ... - EURASNET

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