Cell Nucleus

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Components of the Promoter A) Core promoter. DNA sequence, -1 to -40 bases from the start of the coding DNA. On/Off regulation of the gene regulatory sequence TAF TBP TATA core promoter RNA polymerase RNA start gene It is recognized by a series of DNA-binding proteins: general transcription factors, comprising the pre-initiation complex, including. TBP (tata binding protein) recognizes the nucleotide sequence TATA, about 30 bases back from the start of the gene. TAFs (TBP-associated Factors) part of a group of General Transcription factors accessory proteins necessary for RNA polymerase II 6 Thursday, January 21, 2010
Components of the Promoter B. Proximal promoter (approx. -40 to -150 bases away) regulatory sequence NF1 TAF TBP RNA polymerase gene CAAT GC Proximal promoter TATA RNA start CAAT and GC boxes are bound to transcription factors such as NF1 NF1 recruits a co-activator needed for RNA polymerase to work Whereas the core promoter determines whether or not transcription can take place, the proximal elements regulate frequency of transcription. When methylated by the cell, GC regions inactivate the gene (note, the cell has control, because nothing happens at the core or proximal promoter unless all the transcription factors are present) 7 Thursday, January 21, 2010
Page 1 and 2: Cell Nucleus Gene Structure Control
Page 3 and 4: - Entire genome in each cell. How d
Page 5: Transcriptional Control RNA polyme
Page 9 and 10: Components of the Promoter D) Enhan
Page 11 and 12: The Glucocorticoid Receptor Glucoco
Page 13 and 14: Response Elements on the PEPCK Gene
Page 15 and 16: Question: How do transcription fact
Page 17 and 18: More on Coactivators Coactivators
Page 19 and 20: Histone Acetyltransferase: Step I C
Page 21 and 22: Transcriptional Repression: Histone
Page 23 and 24: DNA Methyltransferases Add methyl
Page 25 and 26: Methylated sites promote gene inact
Page 27 and 28: Main levels of gene expression 1. G

Cell Nucleus

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