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Brazil, (2) Ministério da Agricultura, São Paulo, SP,
Brazil, (3) UFPR, Curitiba, PR, Brazil
P-123 Rapid assay for the on-site detection of potato
pathogens. T. Oberhänsli (1), D. Altenbach (1), P.
Munch (1), K. Maier (1), W. BITTERLIN (1). (1)
BIOREBA AG, Reinach, Switzerland
P-124 Investigating the threat of Phytophthora ramorum to
Ireland: The current situation. J. BRENNAN (3),
D. Cummins (3), S. Kearney (3), J. Choiseul (3),
G. Cahalane (1), S. Nolan (2). (1) Forest Service,
Department of Agriculture, Fisheries & Food,
Dublin, Ireland, (2) Horticulture & Plant Health
Division, DAFF, Maynooth, Co. Kildare, Ireland,
(3) Plant Health Laboratory, Backweston Campus,
Department of Agriculture, Fisheries & Food,
Celbridge, Co. Kildare, Ireland
P-125 Comparison of ELISA, PCR, and a new TaqMan
real-time PCR test for Clavibacter michiganense
subsp. sepedonicus. D. J. GERHARDT (1), A.
Phibbs (2), A. O. Charkowski (1). (1) University
of Wisconsin-Madison, Madison, WI, U.S.A., (2)
Wisconsin Department of Agriculture, Trade and
Consumer Protection, Madison, WI, U.S.A.
P-126 Serological detection of Sweet potato leaf curl virus.
D. L. GUTIERREZ (1), P. Lotrakul (2), N. Murai
(1), R. A. Valverde (1). (1) Dept. of Plant Pathology
and Crop Physiology, Louisiana State University
Agricultural Center, Baton Rouge, LA, U.S.A., (2)
Dept. of Botany, Faculty of Science, Chulalongkorn
University, Bangkok, Thailand
P-127 First detection of the cereal cyst nematode Heterodera
filipjevi in North America. G. YAN (1), R. M.
Smiley (1). (1) Oregon State University, Columbia
Basin Agricultural Research Center, P.O. Box 370,
Pendleton, OR, U.S.A.
P-128 Comparison of techniques used for the detection of
Plum pox virus when using different source material
and sampling time. N. FIORE (2), C. Araya (1),
A. Zamorano (2), F. Gonzalez (2), R. Mora (1),
I. Rosales (1). (1) Instituto de Investigaciones
Agropecuarias (INIA), Santiago de Chile, Chile,
(2) Universidad de Chile, Facultad de Ciencias
Agronómicas, Santiago de Chile, Chile
P-129 Challenges in the management of plant diseases in
the Northern Mariana Islands. D. NANDWANI (1),
J. Tenorio (1). (1) Northern Marianas College
P-130 Detection of Ralstonia solanacearum race 4 in field
samples using a combination of serological and
molecular assays. M. L. PARET (1), R. Kubota (1),
D. M. Jenkins (1), A. M. Alvarez (1). (1) University
of Hawaii at Manoa, HI, U.S.A.
P-131 A multipathogen detection array for virus, viroid,
fungal, and oomycete pathogens of solanaceous
crops. K. L. PERRY (2), B. O. Agindotan (2), N.
Zhang (1), C. Smart (1). (1) Cornell University,
Geneva, NY, U.S.A., (2) Cornell University, Ithaca,
NY, U.S.A.
P-132 Detection and identification of Fusarium species in
field pea roots. F. M. MATHEW (2), T. Barasubiye
(1), S. G. Markell (2), R. S. Goswami (2). (1)
Agriculture and Agri-Food Canada, Ottawa, ON,
Canada, (2) Dept. Plant Pathology, North Dakota
State University, Fargo, ND, U.S.A.
P-133 Efficiency of endoparasitic nematode extraction from
corn roots. J. L. BEHN (1), A. E. MacGuidwin
(2), T. A. Jackson (1). (1) University of Nebraska-
Lincoln, Lincoln, NE, U.S.A., (2) University of
Wisconsin-Madison, Madison, WI, U.S.A.
P-134 A reliable and inexpensive method of nucleic acid
extraction for the PCR-based detection of diverse
plant pathogens. R. Li (2), R. MOCK (2), Q. Huang
(2), J. Abad (1), J. Hartung (2), G. Kinard (2). (1)
USDA-APHIS, Beltsville, MD, U.S.A., (2) USDA-
ARS, Beltsville, MD, U.S.A.
P-135 Synthetic internal control sequences to increase
negative call veracity in multiplexed, quantitative
PCR assays for Phakopsora pachyrhizi. J. S.
HAUDENSHIELD (2), G. L. Hartman (1). (1)
USDA-ARS, Urbana, IL, U.S.A., (2) University of
Illinois, Urbana-Champaign, IL, U.S.A.
P-136 Soybean rust incidence and the response of soybeans
to fungicides in Virginia 2007. P. Phipps (1), E.
STROMBERG (2), S. Rideout (3), D. Holshouser
(1). (1) Virginia Polytechnic Institute & State
University, Suffolk, VA, U.S.A., (2) Virginia
Polytechnic Institute & State University, Blacksburg,
VA, U.S.A., (3) Virginia Polytechnic Institute &
State University, Painter, VA, U.S.A.
P-137 Development of a molecular marker for specific
detection of Fusarium oxysporum f. sp. cubense, a
causing agent of banana wilts disease. P. CHANG
(1), Y. Lin (1), J. Chang (1), E. Liu (1), C. Chao (2).
J. Huang (1). (1) Department of Plant Pathology,
National Chung Hsing University, Taichung City,
Taiwan, Republic of China, (2) Division of Banana
Cultivation and Post-harvest Physiology, Taiwan
Banana Research Institute, Pingtung, Taiwan,
Republic of China
P-138 Detection and differentiation of Potato Cyst
Nematode (PCN) and morphologically similar
species with the NanoChip ® technology. Z. LIU (1),
M. K. Nakhla (1), L. Levy (1). (1) USDA APHIS
PPQ CPHST, Beltsville, MD, U.S.A.
P-139 Development of real-time PCR for the detection
of exotic potyviruses infecting imported plant
germplasm. M. K. NAKHLA (1), K. J. Owens (1),
W. Li (1), L. Levy (1). (1) USDA APHIS PPQ
CPHST, Beltsville, MD, U.S.A.
P-140 Isolation and evaluation of a new race of anthracnose
on dry bean cultivars in North Dakota. R. S.
LAMPPA (1), R. S. Goswami (1). (1) North Dakota
State University, Dept. of Plant Pathology, Fargo,
ND, U.S.A.
P-141 Survey of huanglongbing (HLB) and citrus canker in
the Rio Grande Valley. B. SALAS (1), P. Parker (1).
(1) USDA APHIS, Edinburg, TX, U.S.A.
P-142 Development of real-time PCR for the detection
and identification of potato cyst nematode. M.
K. NAKHLA (1), K. J. Owens (1), W. Li (1), L.
Carta (3), A. Skantar (2), L. Levy (1). (1) USDA
APHIS PPQ CPHST, Beltsville, MD, U.S.A., (2)
USDA ARS Molecular Plant Pathology Laboratory,
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