A proteomic view of probiotic Lactobacillus rhamnosus GG

Review of the Literature
Topic Separation and detection methods Identifi cation method Potentially probiotic strains Reference
bile stress 2-D GE, [ 35S]methionine/cysteine N-terminal sequencing, MALDI-MS P. freundreichii SI41 Leverrier et al., 2003
labeling
bile and acid stress 2-D GE, [ 35S]methionine/cysteine no novel identifi cations P. freundreichii SI41 Jan et al., 2002
labeling
bile, acid, and heat stress 2-D GE, [ 35S]methionine/cysteine LC-MS/MS P. freundreichii SI41 Leverrier et al., 2004
labeling
eff ect of bile stress on cell wall proteome 2-D GE, silver staining MALDI-MS B. animalissubsp. lactis BI07 Candela et al., 2010
eff ect of bile stress on cell-envelope proteome DIGE; SDS-PAGE; SILAC MALDI-MS/MS B. longum NCIMB8809 Ruiz et al., 2009a
exposure to rabbit jejunum 2-D GE, Coomassie staining MALDI-MS L. fermentum I5007 Yang et al., 2007
exposure to rabbit large intestine 2-D GE, Coomassie staining MALDI-MS, nanospray ESI-MS/MS B. longum NCC2705 Yuan et al., 2008
acid stress 2-D GE, Coomassie staining MS B. longum NCIMB8809 Sánchez et al., 2007a
acid stress 2-D GE, silver staining MALDI-MS L. casei Zhang Wu et al., 2011
acid stress 2-D GE, silver staining MALDI-MS L. reuteri ATCC 23272 Lee et al., 2008b
acid stress 2-D GE, silver staining MALDI-MS L. reuteri ATCC 23272 Lee and Pi, 2010
acid stress 2-D GE, [ 35S]methionine/cysteine N-terminal sequencing P. freundreichii SI41 Jan et al., 2001
labeling
heat stress 2-D DIGE MALDI-MS L. gasseri ATCC 33323 Suokko et al., 2008
heat and osmotic stress 2-D GE, [ 35 Table 1. cont.
S]methionine/cysteine N-terminal sequencing L. rhamnosus HN001 Prasad et al., 2003
labeling
oxidative stress 2-D GE, Coomassie staining MALDI-MS B. longum subsp. longum Xiao et al., 2011
BBMN68
comparison of strains with diff erent stress LC LC-MS/MS B. longum NCC2705 Guillaume et al., 2009
tolerance
8
Cell envelope proteome and secretome
cell surface proteins SDS-PAGE LC-MS/MS L. plantarum 423 and WCFS1 Ramiah et al., 2008
L. acidophilus ATCC 4356
cell wall proteins binding to human
2-D GE, Coomassie staining MALDI-MS B. lactis BI07 Candela et al., 2007
plasminogen
cell wall-associated proteome SDS-PAGE (and 2D- GE, silver LC-MS/MS L. salivarius UCC118 Kelly et al., 2005
staining)
surfome SDS-PAGE LC-MS/MS L. plantarum 299v Beck et al., 2009
surfome SDS-PAGE MALDI-MS/MS L. rhamnosus GG Sánchez et al., 2009a
adhesion proteins 2-D GE, Coomassie staining LC-MS/MS L. plantarum 299V Izquierdo et al., 2009
comparison of diff erentially aggregative strains 2-D GE, Coomassie staining MALDI-MS, LC-MS/MS L. crispatus M247 and Mu5 Siciliano et al., 2008