A proteomic view of probiotic Lactobacillus rhamnosus GG

More documents

Recommendations

Info

Review of the Literature Table 2. cont. 16 Bile stress Acid stress Heat stress Protein Lactobacillus Bifi dob. Propionibact. Lactobacillus Bif. Propionib. Lactob. Prop. Classifi cation Name Function 1* 2 3 4 5 6 7 8 9 10 11 12 13 14 7 9 15 16 9 LacZ Beta-galactosidase x Others AglA Probable alpha-1,4-glucosidase x EstC Esterase C x GlmM Phosphomannomutase x Gnt 6-phosphogluconate dehydrogenase x NagA N-Acetylglucosamine-6-phosphate deacetylase x x NagB Glucosamine-6-phosphate isomerase x PhoE Phosphoglycerate mutase family protein x Tal Transaldolase x Tkt Transketolase x x Xfp Fructose 6-phosphate phosphoketolase x x Zwf Glucose-6-phosphate dehydrogenase x x − 2,3-Biphosphoglyceromutase x − 6-Phosphogluconate dehydrogenase x − Formyl-CoA transferase x − HPr kinase/phosphorylase x − Malate dehydrogenase x − Maltosephosphorylase x − Mannitol-1-phosphate-dehydrogenase x − NADPH-dependent aldo- or ketooxidoreductase x − Propable phosphoketolase x − Pyruvate-fl avodoxin oxidoreductase x − UTP-glucose-1-phosphate uridyltransferase x Amino acid metabolism ArgD Acetylornithine aminotransferase x AspA Aspartate ammonia-lyase x CysK Cysteine synthase x x x x DapF Diaminopimelate epimerase x GlnA Glutamine synthetase x IlvC Ketol-acid reductoisomerase x x
Bile stress Acid stress Heat stress Protein Lactobacillus Bifi dob. Propionibact. Lactobacillus Bif. Propionib. Lactob. Prop. Classifi cation Name Function 1* 2 3 4 5 6 7 8 9 10 11 12 13 14 7 9 15 16 9 IlvD Dihydroxy acid dehydratase x IlvE Branched-chain amino acid aminotransferase x x LuxS S-Ribosylhomocysteinase x x MetK S-adenosylmethionine synthetase x x − 3-Phosphoshikimate-1-carboxyvinyltransferase x − Methylmalonyl-CoA mutase large subunit x − Orinithine carbamoyltransferase x − Putative agmatine deiminase x − Saccharopine dehydrogenase related protein x Nucleotide metabolism Adk Adenylate kinase x x CarB Carbamoylphosphate synthase large subunit x Fhs Formate-tetrahydrofolate ligase x GuaA GMP synthase/glutamine amidotransferase x GuaB IMP dehydrogenase x GuaC GMP reductase x PurM Phosphoribosyl-aminoimidazole synthetase x PyrD Dihydroorotate dehydrogenase x PyrG CTP synthase x x PyrH Uridylate kinase x PyrR Bifunctional protein PyrR x − Polyribonucleotide nucleotidyltransferase x x − Putative nucleoside deoxyribosyltransferase x Review of the Literature Transcription and translation Ribosomal proteins RplJ Ribosomal protein L10 x x RplL Ribosomal protein L7/L12 x x RplY Ribosomal protein L25 x RpsA Ribosomal protein S1 x RpsB Ribosomal protein S2 x x x 17
Page 1 and 2: Department of Veterinary Bioscience
Page 4 and 5: CONTENTS Abstract List of abbreviat
Page 6 and 7: ABSTRACT Lactobacillus rhamnosus GG
Page 8 and 9: LIST OF ABBREVIATIONS 2-D DIGE two-
Page 11 and 12: 1. REVIEW OF THE LITERATURE 1.1. Pr
Page 13 and 14: ingestion of Lactobacillus gasseri
Page 15 and 16: 1.1.3. Bifi dobacteria and propioni
Page 17 and 18: Table 1. Proteomic studies of probi
Page 19 and 20: Topic Separation and detection meth
Page 21 and 22: 1.3.1.2. Comparison of growth phase
Page 23 and 24: ecause of the complex nutrient requ
Page 25: Bile stress Acid stress Heat stress
Page 29 and 30: Bile stress Acid stress Heat stress
Page 31 and 32: on the metabolic processes of probi
Page 33 and 34: esistant mutant strain was also abl
Page 35 and 36: Glyceraldehyde-3-phosphate dehydrog
Page 37 and 38: nine proteins that are potentially
Page 39 and 40: down-regulated the chemotaxis syste
Page 41 and 42: 3. MATERIALS AND METHODS All materi
Page 43 and 44: μg of RNA was reverse transcribed
Page 45 and 46: L. rhamnosus GG in MRS medium, and
Page 47 and 48: (EPS) biosynthesis and D-alanylatio
Page 49 and 50: that exports protons from the cell
Page 51 and 52: Results and Discussion 41
Page 53 and 54: 5. CONCLUDING REMARKS AND FUTURE PR
Page 55 and 56: 6. ACKNOWLEDGEMENTS Acknowledgement
Page 57 and 58: Castaldo C., Vastano V., Siciliano
Page 59 and 60: Hickson M., D’Souza A. L., Muthu
Page 61 and 62: Lee K. & Pi K. (2010) Effect of tra
Page 63 and 64: Adaptation and response of Bifidoba
Page 65: Wu R., Wang W., Yu D., Zhang W., Li

A proteomic view of probiotic Lactobacillus rhamnosus GG

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?