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Undergraduate Research Day UMT 2018<br />

Evaluation of Staphylococcus Aureus in Cheeses and Bulk Margarines at<br />

Selected Retail Outlets in Kuala Terengganu.<br />

Nur Nabihah Binti Musa<br />

Supervisor: Dr. Tuan Zainazor Bin Tuan Chilek<br />

Bachelor of Food Science (Food Service and Nutrition)<br />

School of Food Science and Technology<br />

Cheese and bulk margarine are commonly used in cooking and baking among Malaysian.<br />

The temperature abused, cross-contamination during transportation and distribution<br />

process and poor personal hygiene practices among food handlers contribute to existence<br />

of S. aureus. The objective of this study is to investigate the S. aureus contamination in<br />

cheeses and bulk margarine, assess the antimicrobial drug resistance on isolated S.<br />

aureus and to determine the growth rate of isolated S. aureus toward pH (7.0 & 8.0) and<br />

salinity (5%, 10%, 15%, 20%, and 25%). The enumeration and identification of S.<br />

aureus, antibiotic susceptibility testing and the growth rate of S. aureus will be carried<br />

out using selected agar (Baird Parker Agar), identified using disks method and determine<br />

using spectrophotometer respectively. S. aureus will be isolated and identified in cheese<br />

and bulk margarine. It is indicate that mishandling and poor hygiene practice throughout<br />

food supply chain.<br />

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