1810_PeloNews_v1

PELONEWS WITH FOCUS ON CELL CULTURE
PELOBiotech Newsletter Nov/Dec. 2018
Welcome dear Scientist,
the end is near, I can feel it, and yes I can see it, too, summer
is over, oooo, but: yephee, ski season is coming. And as we
have celebrated a great teenie birthday party with our partner
baseclick, we just continue partying: So get a good deep
look at our Offers this time. We might just have right tools for
you and your project and ...maybe we have even the
right cell for your project. Cheers!
Christiane Büchsel
Editor in Chief PELONews
"If you are going
through hell,
keep going!“
-Winston Churchill --
Content
Happy Birthday…
baseclick...
Meet us at… TEDD
NEW Products by
Funakosi
Noviocell
abc biopply
Happy Birthday …. 10 years baseclick. The
Neurieder Biotech company, famous for its
Great meeting at
click chemistry Assays, celebrated at Literaturhaus
in Munich. CEO Thomas Frischmuth
EACR in Berlin
was in best festive mood.
Cell Biologics
Cell Systems
Featured Fields
3D
Want to know
more about PELO-
Biotech? Visit our
site and find out
about our Cell Culture
Fall-Offers.
You like what you see? Sign up for PELO-
News now at www.pelobiotech.com or just
fill out the following form here.
And if you are already a fan
-> Join PeloClub here and save every time
you order.
Cancer
Primary Cells
Dyes
Aged cells

PELONews
About
Noviocell
Pics. Noviocell
Noviocell BV is a
highly innovative
biotech company
headquartered in
Oss, The Netherlands.
It focuses on
the development
of the first ever
synthetic solution
for 3D cell culturing,
ranging
from organoid
growth to personalized
medicine.
Noviogel acts as novel wound dressing
Full-thickness dorsal skin wounds in mice were treated with Noviogel for 3 and 7 days. No foreign body
reaction, but even a significant decrease in granulocyte population in the wound was observed. Noviogel
acts as a novel wound dressing, because it gelates upon contact with the human body, and remain in
place without any additional support. Furthermore, it can be easily and painless applied and removed
because of its reversible thermosensitive behavior.
Reference: Roel C. op ‘t Veld et al. Biomaterials, 2018:Vol. 181, p 392-40.
Noviogel
Noviogel is a bioelastic reversible thermosensitive synthetic solution for 3D Culture based on Polyisocyanopeptide
(PIC) hydrogel, a new class of advanced polymers. PIC hydrogel is an improved platform for cell studies, because it
combines the unique benefits of natural and synthetic biomaterials. PIC hydrogels uniquely perform like collagen,
while possessing the same characteristics as other commercial hydrogels.
fully reversible thermo sensitive and cells can be easy recovered
Syntrix
Noviocell Syntrix is a synthetic, bioactive cell culture matrix based on self-assembling hydrogel technology. Gels
form rapidly and spontaneously under physiological conditions from appropriate precursor solutions, allowing
mild encapsulation of cells.
presenting a low-adhesion surface to cells
enhance cell adhesion and performance

New @ Cell
Biologics
Also check out ->
Human Primary
cells of Smoker´s:
just check the
Human cell list.
Aged Mouse & Rat cells
Our partner Cell Biologics has a specialty now. They offer Aged
Mouse Cells (25-78 week old). You can customize your Cell
BKS db Mouse Cells
and control cells ->
Just check the Animal
product list.
Just click to get one
of the broadest
portfolios of Animal
and/or Human
Cells here or just
scan the QR-code
to request your personal
copy here.
Look at this: Aged Mouse Aortic Endothelial Cells
Aged Mouse Aortic Endothelial Cells are isolated from aorta of pathogen-free laboratory mice of 58-78 weeks. A-
ged Mouse Aortic Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating
solution for 2 min and incubated in Cell Biologics’ Culture Complete Growth Medium generally for 3-7 days. Cultures
are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials.
Each vial contains at least 1x10 6 cells per ml and are delivered frozen.
The method we use to isolate primary endothelial
cells was developed based on a
combination of established and our
proprietary methods.
These cells are pre-coated with PECAM-1
antibody, following the application of magnetic
pre-coated with secondary antibody.
Find out more about all the other aged
cells here.

PELONews
3D CoSeedis TM : The
Micro-Organoid Histology System
The micro-organoid histology system: all components
for successful paraffin embedding are
included.
Pic : abc biopply
Our partner abc biopply has
recently released its new
micro-organoid histology
system, being the latest extension
to abc biopply’s 3D
CoSeedis technology
platform.
The micro-organoid histology
system offers a validated
and standardised work process
form the generation of
spheroidal or nonspheroidal
3D cell aggregates
to paraffin embedded
micro-arrays of 3D cell structures.
Detailed protocols
take the researcher through
each step in a comprehensible and reproducible way.
The system includes all required components for successful paraffin embedding
and therefore guarantees an easy entry point to IHC on organoids.
The micro-organoid histology system is fully compatible with other
products from the 3D CoSeedis product line and can therefor be integrated
into already existing workflows
without effort.
Contact us or Marco Leu, Co-Owner abc
-biopply, for further information and
protocols.
Meet us
at… TEDD
This year’s TEDD Annual
Meeting brings together experts
from diverse fields
with a shared interest in
advanced 3D models. Join
our Swiss partner abc biopply
and Dr. Peter Frost, CEO
PELOBiotech, for this meeting
to celebrate another
fruitful collaboration year
with new perspectives
ahead.
And if you haven´t seen 3D
CoSeedis in action so far,
here is your best opportunity
to ask Peter und Marco
personally.
Organised by ZHAW Institut
für Chemie und Biotechnologie
-> Campus Grüental, GA
203 & Kalthaus GC 181,
8820 Wädenswil
-> 25. October 2018
Check out
our NEW
3D Flyer
Pic: Marco Leu
Step-by-step protocols take the researcher from cell aggregation all the way to a
paraffin-embedded micro-tissue arrays of 3D cell structures.
According to Andreas R. Thomsen et al., Lab Chip, 2018, 18, 179 - 189
Pic. abc biopply

Pic. Funkoshi
Check out our NEW Products:
NIPRO's Antibody Immobilization Bag A
When stimulating cells by antibodies, antibodies are immobilized on cell culture microplate. However, this method
involves hard work if many cells are required to be stimulated on many microplates should be used.
NIPRO's Antibody Immobilization Bag A saves cost and time.
Just put antibody solution, antibodies are immobilized on inside of the bag and after that, cells can be cultured in
this closed bag system.
This bag has excellent gas permeability; just place it in CO 2 incubator.
Features
High gas permeability
High safety
High transparency
Needleless support * Please use lock type syringe Curious?
Read more and download your flyer here.
Ask for our extensive product list of Dyes and TR-FRET: sales @pelobiotech.com
PolyamineRED
PolyamineRED is the world's first reagent for detecting intracellular polyamines in live cellswithout any pretreatment
and cell lysis.
This TAMRA (tetramethylrhodamine)-conjugated derivative of glycine propagyl ester specifically reacts with linear
primary alkylamine and has cell-penetrating properties, specifically reacts with polyamines inside the cells and
labelled polyamines with red fluorescent dye TAMRA.
Read more here.
Features
Specifically labels polyamines
Can be detected by filter set for Rhodamine (Ex./Em. = 560 / 585 nm)
Cell permeable : compatible with live cell imaging
No pretreatments required
Can do semi-quantification of total polyamines amount (*) by intracellular fluorescence intensity.
* Only total polyamines, not for each polyamine.

PELONews
World's first Acrolein Detection Reagent in Live Cells
Acrolein, which is found in a common dietary and environmental pollutant, is known as a highly toxic metabolite
for cells. Not only in outer environment, but also acrolein is endogenously gererated in cells,
especially under the oxidative stress condition.Acrolein has being researched for more than half century and many
detection methods were developed, such as fluorometric method, mass spectromy or antibody based detection
method, etc. However, conventional methods are not appropriate for the detection in live cells and show poor selectivity
and sensitivity.
Our AcroleinRED is the world first cell based acrolein detection reagent in live cells condition without
any pre treatment and cell lysis.
This reagent is based on novel phenylazide acrolein click chemistry discovered by Drs. Katsunori Tanaka and Ambara
Pradipta. AcroleinRED specifically react with either extracellular acrolein conjugate released from cell surface
lipids or intracellular acrolein generated via enzymatic pathway and label acrolein with TAMRA fluorophore. More
infos here.
Pic. Funkoshi.
Antibody-free Primary Cells
PELOBiotech partner Cell Systems has recently been selected to supply antibody
-free human primary cells for a pioneering manufacturer of 3D organ-on-a-chip
devices. Industry leaders in the 3D- and microfluidic device field are now providing
high-quality cells with their chips, to give researchers the best shot at a viable
culture and successful experiment.
Cell Systems human primary cells were selected for their
antibody-free isolation process, viability, consistency, and
deep inventory. We are proud to provide these high-quality
primary cells to our customers in the EU!
How to
reach us:
If you need any further
assistance or if you like
what you see, tell us:
PELOBiotech GmbH
Klopferspitz 19
82152 Planegg | Germany
Tel.: +49 89 517286 59 0
info@pelobiotech.com
www.pelobiotech.com
HRB 197203
Dr.Peter Frost
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