TRISOMY 8 MOSAICISM: CELL CYCLE KINETICS AND ...
TRISOMY 8 MOSAICISM: CELL CYCLE KINETICS AND ...
TRISOMY 8 MOSAICISM: CELL CYCLE KINETICS AND ...
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ABSTRACT<br />
<strong>TRISOMY</strong> 8 <strong>MOSAICISM</strong>: <strong>CELL</strong> <strong>CYCLE</strong> <strong>KINETICS</strong> <strong>AND</strong><br />
DISTRIBUTION OF <strong>TRISOMY</strong> 8 <strong>AND</strong> NORMAL <strong>CELL</strong>S IN<br />
EMBRYONIC <strong>AND</strong> EXTRA-EMBRYONIC TISSUES<br />
Bonnie J Pettit<br />
Amniocentesis identified a fetus with trisomy 8 in one third of metaphase cells<br />
while remaining cells were normal. The purpose of this project was to determine the<br />
level of trisomy 8 mosaicism within embryonic and extra-embryonic tissues and cell<br />
cycle division rates for the two cell lines. Tissue samples collected at birth included cord<br />
blood, cord tissue, peripheral blood, and placental tissues. The level of trisomy 8 and<br />
normal cells was determined in G-banded metaphases and fluorescence in situ<br />
hybridization of interphase cells. Trisomy 8 metaphase and interphase cell investigations<br />
identified a significant decrease of trisomy 8 cells undergoing mitosis. Cell cycle kinetics<br />
was measured using incorporation of bromodeoxyuridine during 48 hours to determine<br />
the number of cell divisions in metaphase cells. There was no evidence suggesting a<br />
significant difference in growth rates using Chi-squared goodness of fit test.
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