TRISOMY 8 MOSAICISM: CELL CYCLE KINETICS AND ...

When comparing embryonic tissues, cord blood, transformed blood, peripheral
blood, and umbilical cord, the percentage of trisomy 8 cells were within 4% of each
other, excluding the transformed blood, which resulted in 0% trisomy 8 cells. Since
different cells are stimulated in the peripheral and cord blood (T-cells) than the
transformed blood (B-cells), the data suggests the T-cells are more tolerant of the extra 8
chromosome or perhaps the path of the lymphoblast lineage is involved. Between the
extra-embryonic tissues, the amnion had a higher number of trisomy 8 cells than the
chorion and villi in the metaphase spreads.
The goal for FISH interphase counts was 500 cells in each sample (Table 2). The
interphase cells were scored as a normal male with two red (8) and one green (X) signal
(figure 3) and as trisomy 8 with three red (8) and one green (X) signal (figure 4).
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