Controlled Environment Systems for High ... - Bioscience Tools

Perfusion Chambers
Small Volume Perfusion Chamber for 10mm Coverslips Consists of
two parts. The bottom part has a special profile to fit No 2, 10 mm O.D., coverslip. The top part has 8 mm
round working compartment with slanted edges for easy access to your sample and two separate compartments
for solution inflow and outflow. This prevents bubbles from coming into the chamber. Item#:
CSC-10P
• Outside diameter: 35mm • Working volume:
00 microl
mm diameter, approx.
Laminar Profile Perfusion Chamber Polycarbonate chamber with laminar cutout to
provide smooth perfusion. Permanently attached to No .5 glass coverslip for use during imaging. Thin
3mm profile allows you to use water immersion optics and access samples for recordings, injection and
local substance application. Separate inflow and outflow compartments prevent bubbles from entering the
working volume during perfusion. Incorporated channel for reference electrodes or temperature probes.
Optional accessories: a mounting adapter PLD-A and a microscope adapter MA. Consider adding an
outflow unit for suction to remove solution during perfusion. Item#: PLD22x40
Adapter for Laminar Perfusion Chamber This adapter allows you to use laminar
flow chamber PLD22x40 with microscope adapters MA or IMA, which can be used to mount miniature
holders for different accessories. Comes with for sets of clamps and mounting thumb screws to fix the
chamber. Item#: PLD-A
Low Profile Small Volume Laminar Perfusion Chamber for Coverslips,
PCCS2
Inside diameter is only 10 mm. The chamber has two separate openings for solution inflow and outflow
to provide smooth perfusion and prevent bubbles from coming in. Low profile permits easy access with
electrodes, pipettes and immersion optics. Combined with miniature holders, forms a perfusion system.
Easy to use, simply release adhesive layer on the coverslip (even with media still present), and attach the
chamber from the top. Coverslip is flashed on the bottom of the chamber.
The chamber is made out of polycarbonate. It is only 5.5 mm thick (high). The bottom of the chamber is
a replaceable 30mm round coverslip. The coverslip is attached to the chamber by using adhesive layers
(or any adhesive, even melted wax). Easy to clean after use. Note: Can be used with any thickness 30mm
coverslips.
The chamber fits inside microscope stage adapter, specify microscope model when ordering. The chamber
can be rotated inside the stage to provide required orientation of the sample. Custom stages for non-standard
microscopes are available upon request.
Screw-type or magnetic tubing/electrode holders extend this flexibility even further. They can be positioned
anywhere around the chamber, so that tubing is not in the way of objectives or recording electrodes.
Furthermore, the holders can adjust the angle and, as a result, tubing can approach the sample from any
direction. The holders fit to perfusion manifolds. They also can be used to position reference electrodes
or temperature probes, or even tubing to deliver gases, for example oxygen, over the sample. MTH-S
stainless suction tubing provides smooth perfusion. If you are planning to use this system during perfusion
experiments, all you need is a outflow unit for suction to remove solution during perfusion. Can be
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