Controlled Environment Systems for High ... - Bioscience Tools

Call 1-877-853-9755
The majority of available perfusion systems have long lines of tubing, which have to be filled before con-
ducting experiments. However, sometimes applied substances are available only in limited quantities or are
extremely expensive, prohibiting the usage of conventional perfusion systems. This system utilizes small
plastic tubes with conical bottom and thin Teflon tubing to connect to your system. It can be used with as
little as 00 µl volumes of solution. Tubes with solutions are simply threaded into the holder. Solutions are
easy to refill during the experiment. Can be used with miniature manifolds. Uses pressure source built-inside
the controller to deliver the solutions, unless the solutions are withdrawn by a negative pressure like
in controlled flow system or intracellular perfusion setup. The system comes with all necessary tubing and
fitting to connect to a single pressure source. The small size of 91 x 48 x 17 mm (without tubing attached)
allows to position solutions near your sample. Can be attached to a ft. rod (included). Comes with X-block
to attach onto a standard 0.5 in. stand. The body has 6.5 mm I.D. opening, which allows to mount the system
on a micromanipulator. Includes 6 plastic replaceable tubes (2 and 3.5ml).
Includes Miniature Manifold for Single Cell Superfusion
Miniature manifolds are used to achieve fast solution application to small samples (single cells, for example).
Due to the small size of the sample, solution exchange around the sample can happen in as fast as
few ms. The miniature manifolds also offer advantage of not contaminating the whole bath chamber, but
applying the substances locally (provided that perfusion chamber is used with frequent wash). Comes with
three replaceable threaded tips of 360, 250 and 100 micron I.D. Incorporates 2 ft. long Teflon tubing for direct
connection to perfusion systems. Inside tubing is easy to wash. The threaded replaceable tips are washable
and allow one to use the manifold with solutions under higher pressure. The length of tips is around
in., which can be cut to the required length. Incorporates 10cm long 4mm O.D. rod to mount on miniature
manipulators and 7mm O.D. adapter to mount on regular manipulators. Incorporates eight separate channels.
If used with less number of solutions, multiple channels are filled with the same solution, which accelerates
washout and delivery. Includes two nozzles to use with small volume chambers as a regular manifold (the
rod fits inside magnetic holders MTH).
Includes Flow Control Unit, up to 22 ml/min Suction
Comes with a set of tubing for different flow ranges up to 22 ml/min. Includes a DC power supply. This high
flow rate unit can be used for solution suction/aspiration from open perfusion chambers. The unit is configured
as an analog option for automatic outflow when perfusion is turned ON.
16-channel Valve Controller
The included 6-channel controller can operate in AUTO Memory mode, when a set of chosen valves opens
automatically at the time the other valves close. This is useful for automatic switching to preset solutions
after the test solution is OFF, and provides uninterrupted solution flow.
INHIBIT mode with manual and external controls allows you to switch all channels OFF at once, if required.
The controller has an option for valves control by Binary Encoding using only 2-4 digital inputs, in case if
a limited number of digital outputs are available in your system. The RS232 port allows automation of solution
switching and integration with imaging systems.
ANALOG output can be configured to monitor channel switching during manual operation. By default,
ANALOG output is used to switch an outflow unit ON automatically every time perfusion is ON.
7