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Algae Anatomy, Biochemistry, and Biotechnology

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Lambertian Surfaces . . .............................................. 198<br />

Units Conversion. . . . . .............................................. 199<br />

Radiant <strong>and</strong> Luminous Flux (Radiant <strong>and</strong> Luminous Power) . ................ 199<br />

Irradiance (Flux Density) . . . . ....................................... 200<br />

Radiance. ...................................................... 200<br />

Radiant Intensity . . . .............................................. 200<br />

Luminous Intensity . .............................................. 200<br />

Luminance . . . .................................................. 200<br />

Geometries . . . .................................................. 200<br />

PAR Detectors . . . . . . .............................................. 200<br />

Photosynthesis–Irradiance Response Curve (P versus E curve) . . ................. 202<br />

Photoacclimation. . ................................................... 205<br />

Suggested Reading . . ................................................. 206<br />

Chapter 6<br />

Algal Culturing . ....................................................... 209<br />

Collection, Storage, <strong>and</strong> Preservation . . .................................... 209<br />

Culture Types ....................................................... 211<br />

Culture Parameters ................................................... 213<br />

Temperature . ..................................................... 213<br />

Light. . . ......................................................... 213<br />

pH ............................................................. 213<br />

Salinity . ......................................................... 214<br />

Mixing.......................................................... 214<br />

Culture Vessels . . . ................................................... 214<br />

Media Choice <strong>and</strong> Preparation ........................................... 215<br />

Freshwater Media . . . . .............................................. 216<br />

Marine Media ..................................................... 225<br />

Seawater Base . .................................................. 225<br />

Nutrients, Trace Metals, <strong>and</strong> Chelators . . ............................... 226<br />

Vitamins . ...................................................... 227<br />

Soil Extract . . . .................................................. 227<br />

Buffers........................................................ 228<br />

Sterilization of Culture Materials . . . . . .................................... 235<br />

Culture Methods . . ................................................... 236<br />

Batch Cultures. .................................................... 237<br />

Continuous Cultures . . .............................................. 239<br />

Semi-Continuous Cultures . . .......................................... 241<br />

Commercial-Scale Cultures . .......................................... 241<br />

Outdoor Ponds .................................................. 241<br />

Photobioreactors . . . .............................................. 243<br />

Culture of Sessile Microalgae . . . ...................................... 244<br />

Quantitative Determinations of Algal Density <strong>and</strong> Growth . ..................... 244<br />

Growth Rate <strong>and</strong> Generation Time Determinations . . . ....................... 249<br />

Suggested Reading . . ................................................. 249<br />

Chapter 7<br />

<strong>Algae</strong> <strong>and</strong> Men . ....................................................... 251<br />

Introduction . ....................................................... 251<br />

Sources <strong>and</strong> Uses of Commercial <strong>Algae</strong> .................................... 252

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