Leica TCS SP5 - ZMBH

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1.2 About this Operating Manual Whenever this manual refers to the "system" or provides no specific information for one of the systems, then the notes, instructions and information applies to TCS SP5 and TCS SP5 X. The main focus of this operating manual is directed to the safety notes that must be strictly adhered to when working with the Leica TCS SP5 and Leica TCS SP5 X. In addition, this operating manual provides a rough overview of the operating principle of laser scanning microscopes. It presents you with the first steps for activating and commissioning the system and provides important information about the Leica Application Suite Advanced Fluorescence (LAS AF) software. The system is delivered with the latest version of the licensed LAS AF. To maintain information on the most current level, the description of software functions was intentionally omitted from this operating manual. Instead, reference is made to the online help of the LAS AF in which you can find up-to-date explanations and instructions for the corresponding software functions. Please read the chapter "Introduction to the LAS AF" in this operating manual to familiarize yourself with its setup and operation. Additional information about specific functions can then be found in the online help. 10
2. Leica TCS SP5 / TCS SP5 X 2.1 TCS SP5 system overview Figure 1: TCS SP5 system components (overview) 1 TCS SP5 Scanner 2 Main switch board 3 TCS workstation 4 Supply unit 5 Control panel 6 Microscope 11
Page 1 and 2: Leica TCS SP5 Leica TCS SP5 X User
Page 3 and 4: Table of Contents 1. General ......
Page 5 and 6: 6.3.3 VIS and UV Systems with Inver
Page 7: 11.3 Cleaning the Microscope Surfac
Page 12 and 13: 2.2 TCS SP5 X system overview Figur
Page 14 and 15: 2.5 Patents The Leica TCS SP5 produ
Page 16 and 17: 2.7.1.3 TCS SP5 X with inverted mic
Page 18 and 19: 2.7.3 Requirements Regarding Ambien
Page 20 and 21: 20 The components for laser safety
Page 22 and 23: 2.8.3 Overview of Usable VIS/UV Las
Page 25 and 26: 3. Safety Instructions and their Me
Page 27 and 28: 4. General Safety Instructions 4.1
Page 29 and 30: 4.4 Safety Instructions for the Use
Page 31 and 32: 5. Safety Devices 5.1 Disconnecting
Page 33 and 34: The key switch for protection again
Page 35 and 36: Immediately disconnect the system f
Page 37 and 38: 5.6 Remote Interlock Connections on
Page 39 and 40: 5.9 Special Laser Safety Equipment
Page 41 and 42: 5.10 Safety labels on the system Th
Page 43 and 44: 5.10.2 Upright microscope DM 5000/6
Page 45 and 46: 5.10.3 Scan Head Angled front view
Page 47 and 48: 5.10.5 External UV laser 4 Figure 2
Page 49 and 50: 5.10.7 MP beam coupling unit Angled
Page 51: 5.10.9 Mirror Housing Front view of
Page 54 and 55: 54 Do not introduce any reflective
Page 56 and 57: 6.5 Changing Specimens 56 Never cha
Page 58 and 59: 6.7 Changing the Transmitted-Light
Page 60 and 61:
6.8 Mirror housing on upright micro
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6.9 Changing Filter Cubes, Beam Spl
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Figure 41: Spacer on objective 64 P
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66 Use your personal user ID if one
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68 The power supply of the achromat
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7.2 Starting the LAS AF 70 With the
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7.3 Setting Up Users 72 The default
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74 6. Shut down the computer. On th
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9.2.2 Accessing the Online Help The
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9.3 Structure of the graphical user
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10. Introduction to Confocal Work 1
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1 Filter cube 2 Specimen 3 Objectiv
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10.1.3 Why Scan? Specimens must be
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Out of focus in focus 1 2 3 Pinhole
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work is required. Once all paramete
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Figure 67: Selecting the illuminati
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Exc Refl Exc1 Exc2 Em 1 Em 2 Em Fig
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Figure 71: Optical sections with a
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Numerical Aperture 1,4 1,25 0,4 Opt
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Figure 74: Zoom, Pan and Rotation c
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extremely sensitive specimens and i
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Figure 78: Simultaneous scan of two
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dominant, the selection of a differ
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thicknesses between 0.5 µm and 2.5
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10.4.5.3 Orthogonal Projections A f
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imposed here by a number of factors
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(measuring parameters) does not in
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11. Care and Maintenance 11.1 Gener
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12. Transport and Disposal 12.1 Cha
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Curvature of Image Field The curved
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Lambda Series Stack of individual i
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Dry Objective A microscopic objecti
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125
Page 127 and 128:
127
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15.2 Declaration of conformity 129
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131
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Leica TCS SP5 - ZMBH

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