Leica TCS SP5 - ZMBH

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2.8.3 Overview of Usable VIS/UV Lasers for TCS SP5 X The Leica TCS SP5 X features a combination of the lasers listed below. 22 Laser type Wavelength [nm] Maximum luminous power at laser output [mW] Diode 405 405 < 120 < 7 Diode 405 p 405 < 5 (mean power) UV, external 355 < 500 < 18 Ar 488 solid-state laser Achromatic light laser 458, 476, 488, 496, 514 < 200 < 50 488 < 470 < 70 Maximum luminous power in focal plane [mW] < 0.3 (mean power) Pulse duration Continuous wave (cw) pulsed, 60 ps 470 – 670 < 500 < 50 Pulsed Table 4: Usable lasers for TCS SP5 X Continuous wave (cw) Continuous wave (cw) Continuous wave (cw) The components for laser safety are designed only for the laser variants listed.
2.8.4 Which Laser Class Does the Product Have? Laser variant VIS UV MP Wavelength range Configuration Laser class 400 - 700 nm, (visible laser radiation) 350 - 700 nm, (visible and invisible laser radiation) 350 - 1600 nm, (visible and invisible laser radiation) 2.8.5 Required Laser Safety Measures Combination of lasers from Chapter 2.8.3 or 2.8.1 (without lasers having wavelengths of 350 - 400 nm) Combination of lasers from Chapter 2.8.3 or 2.8.1 (VIS and UV lasers) Combination of lasers from chapter 2.8.1 (VIS/UV Laser), chapter 2.8.2 (IR Laser) or chapter C2.8.3 (VIS/UV Laser) 3B / IIIb 3B / IIIb 4 / IV Please observe the laser safety measures for laser class 3B / IIIb (VIS and UV systems) or laser class 4 / IV (MP systems) in accordance with applicable national and federal regulations. The owner/operator is responsible for observing the laser safety regulations. 23
Page 1 and 2: Leica TCS SP5 Leica TCS SP5 X User
Page 3 and 4: Table of Contents 1. General ......
Page 5 and 6: 6.3.3 VIS and UV Systems with Inver
Page 7: 11.3 Cleaning the Microscope Surfac
Page 10 and 11: 1.2 About this Operating Manual Whe
Page 12 and 13: 2.2 TCS SP5 X system overview Figur
Page 14 and 15: 2.5 Patents The Leica TCS SP5 produ
Page 16 and 17: 2.7.1.3 TCS SP5 X with inverted mic
Page 18 and 19: 2.7.3 Requirements Regarding Ambien
Page 20 and 21: 20 The components for laser safety
Page 25 and 26: 3. Safety Instructions and their Me
Page 27 and 28: 4. General Safety Instructions 4.1
Page 29 and 30: 4.4 Safety Instructions for the Use
Page 31 and 32: 5. Safety Devices 5.1 Disconnecting
Page 33 and 34: The key switch for protection again
Page 35 and 36: Immediately disconnect the system f
Page 37 and 38: 5.6 Remote Interlock Connections on
Page 39 and 40: 5.9 Special Laser Safety Equipment
Page 41 and 42: 5.10 Safety labels on the system Th
Page 43 and 44: 5.10.2 Upright microscope DM 5000/6
Page 45 and 46: 5.10.3 Scan Head Angled front view
Page 47 and 48: 5.10.5 External UV laser 4 Figure 2
Page 49 and 50: 5.10.7 MP beam coupling unit Angled
Page 51: 5.10.9 Mirror Housing Front view of
Page 54 and 55: 54 Do not introduce any reflective
Page 56 and 57: 6.5 Changing Specimens 56 Never cha
Page 58 and 59: 6.7 Changing the Transmitted-Light
Page 60 and 61: 6.8 Mirror housing on upright micro
Page 62 and 63: 6.9 Changing Filter Cubes, Beam Spl
Page 64 and 65: Figure 41: Spacer on objective 64 P
Page 66 and 67: 66 Use your personal user ID if one
Page 68 and 69: 68 The power supply of the achromat
Page 70 and 71: 7.2 Starting the LAS AF 70 With the
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7.3 Setting Up Users 72 The default
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74 6. Shut down the computer. On th
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9.2.2 Accessing the Online Help The
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9.3 Structure of the graphical user
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10. Introduction to Confocal Work 1
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1 Filter cube 2 Specimen 3 Objectiv
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10.1.3 Why Scan? Specimens must be
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Out of focus in focus 1 2 3 Pinhole
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work is required. Once all paramete
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Figure 67: Selecting the illuminati
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Exc Refl Exc1 Exc2 Em 1 Em 2 Em Fig
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Figure 71: Optical sections with a
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Numerical Aperture 1,4 1,25 0,4 Opt
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Figure 74: Zoom, Pan and Rotation c
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extremely sensitive specimens and i
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Figure 78: Simultaneous scan of two
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dominant, the selection of a differ
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thicknesses between 0.5 µm and 2.5
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10.4.5.3 Orthogonal Projections A f
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imposed here by a number of factors
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(measuring parameters) does not in
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11. Care and Maintenance 11.1 Gener
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12. Transport and Disposal 12.1 Cha
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Curvature of Image Field The curved
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Lambda Series Stack of individual i
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Dry Objective A microscopic objecti
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125
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127
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15.2 Declaration of conformity 129
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131
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Leica TCS SP5 - ZMBH

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