Neurokinin Bs and neurokinin B receptors in zebrafish- potential role ...

A
1 tatatctaaatatttctggacatttctggcatggcacagtcacagaacggatctaaccta
M A Q S Q N G S N L 10
61 acggggaactttacgaaccagttcgtgcagccgccgtggcgcgtggcgctgtggtcggtg
T G N F T N Q F V Q P P W R V A L W S V
121 gcgtacagctccatcctggcgatcgcggtgttcgggaatctgatcgtcatgtggatcatt
A Y S S I L A I A V F G N L I V M W I I 50
181 ctggctcataagcggatgcgaaccgtcaccaactactttctgctcaacctggcgttttcg
L A H K R M R T V T N Y F L L N L A F S
241 gacgcctccatggccgccttcaacactttgatcaatttcgtttacgccacacacggagat
D A S M A A F N T L I N F V Y A T H G D 90
301 tggtatttcggagaagcctactgcaaatttcacaactttttccccgtcacctccgtgttt
W Y F G E A Y C K F H N F F P V T S V F
361 gccagcatttactccatgagcgcaatcgcagtcgacaggtacatggccatcatccatcct
A S I Y S M S A I A V D R Y M A I I H P 130
421 ctgaaaccacgactctcggcgacggccaccaaagtgatcattgtgtgtatctgggtgctc
L K P R L S A T A T K V I I V C I W V L
481 gctgtggttttggccttcccgctgtgtttcttttcaaccatcaaaaaactgcccaaacga
A V V L A F P L C F F S T I K K L P K R 170
541 actctctgctatgttgcctggccgagaccttcagaagaccctttcatgtatcatatcatt
T L C Y V A W P R P S E D P F M Y H I I
601 gtggcgatgctggtgtatgttctgccgctggtggtcatgggtatcaactacactattgtc
V A M L V Y V L P L V V M G I N Y T I V 210
661 ggattgaccctttggggaggagagattcctggtgactcctcagacaactatcagggccag
G L T L W G G E I P G D S S D N Y Q G Q 230
721 ctcagggccaagaggaaggtggtgaaaatgatgatcattgtagtggtgacctttgccttc
L R A K R K V V K M M I I V V V T F A F
781 tgctggctgccgtaccatgtgtatttcctggtgacgggattgaacaagcagctggctcga
C W L P Y H V Y F L V T G L N K Q L A R 270
841 tggaagttcattcagcagatctatctgtccatcatgtggcttgccatgagctccaccatg
W K F I Q Q I Y L S I M W L A M S S T M
TM7
901 tataaccccattatttactgctgcctaaacagccggtttcgcgctggcttcaaacgtgtt
Y N P I I Y C C L N S R F R A G F K R V 310
961 ttccgctggtgcccttttgtgcaagtctctgactatgacgagcttgagctgcgggctatg
F R W C P F V Q V S D Y D E L E L R A M 330
1021 aggcataaagtagcgcggcagagcagcatgtacacaatgtcacgaatggagaccaccgta
R H K V A R Q S S M Y T M S R M E T T V 350
1081 gtcaccgtgtgtgacccatcagagccaaacacccagccaggccggaagagcctgcttaac
V T V C D P S E P N T Q P G R K S L L N 370
1141 caccaccaccaccacaacggctgctccaacccagccaagagcaaagaaataacatacatg
H H H H H N G C S N P A K S K E I T Y M 390
1201 caaagcgacccgaaggaggaattctcctgagaaggacttttgatgtaagattcacac
Q S D P K E E F S * 399
1261 tgaagcattaag
TM4
TM3
TM6
TM5
TM2
TM1
30
70
110
150
190
250
290
B
1 tttaagaaggatttcacggttaaatctaccatggctggtcctcagagcggctcaaatgtg
M A G P Q S G S N V 10
61 acgcgtaatttcacaaatcagttcgtgcagccgccgtggcgggtcgccgtctggtcggtc
T R N F T N Q F V Q P P W R V A V W S V
121 gcttacagctcggtgctcgcggtcgccgtgttcggaaacctcattgttatttggatcatt
A Y S S V L A V A V F G N L I V I W I I 50
181 ttggcccataaacggatgcgcaccgtcaccaactattttttgctcaacctggcgttttcc
L A H K R M R T V T N Y F L L N L A F S
241 gacgcgtccatggccgccttcaacacgctcatcaacttcatttacgccacgcacggagag
D A S M A A F N T L I N F I Y A T H G E 90
301 tggtacttcggagaggtttactgcaagttccacaacttcttccctgtgaccgccgtgttt
W Y F G E V Y C K F H N F F P V T A V F
361 gccagcatttactccatgacagcgattgcagtcgacaggtacatggccataatacatcct
A S I Y S M T A I A V D R Y M A I I H P 130
421 ctgaagcctcgtctgtcagccacggctactaaagtggtgattgtctgtatttgggcactg
L K P R L S A T A T K V V I V C I W A L
481 gcagtgattttggctttcccgctgtgtttctactccaccacgagaaccatgcctcgcaga
A V I L A F P L C F Y S T T R T M P R R 170
541 accatttgctacgtcgcctggccaagaccggctgaggattcattcatgtatcacatcata
T I C Y V A W P R P A E D S F M Y H I I
601 gtgacggtgctggtctacatgctgcccctagtggtgatgggcatcacctacactatagtc
V T V L V Y M L P L V V M G I T Y T I V 210
661 ggggttacactttggggaggagagattcctggagactcgtcggacaattatgttggacag
G V T L W G G E I P G D S S D N Y V G Q 230
721 ctacgtgctaagaggaaggtggtgaagatgatgatcgtggtggtggtgactttcgccctc
L R A K R K V V K M M I V V V V T F A L
781 tgctggttgccgtatcacatctatttcatcgtaacaggcctgaacaaacgcctgaacaag
C W L P Y H I Y F I V T G L N K R L N K 270
841 tggaagtccatccagcaggtgtatctgtctgtgctgtggctggccatgagctccaccatg
W K S I Q Q V Y L S V L W L A M S S T M
TM7
901 tacaaccccatcatttactgctgtctgaatggcagatttcgcgcgggcttcaagcgggcc
Y N P I I Y C C L N G R F R A G F K R A 310
961 ttcaggtggtgtcccttcattcaggtgtccagctatgacgaactggaactccgtcccacc
F R W C P F I Q V S S Y D E L E L R P T 330
1021 cggctccatccacgcaaccagagcagcatgtgcaccctgtcccgcgtcgacaccagcctc
R L H P R N Q S S M C T L S R V D T S L 350
1081 catggtgaggacccacgacgcagtcagcggaagagcaccaaatcccaatgtctggtggag
H G E D P R R S Q R K S T K S Q C L V E 370
1141 gtcagagacgaaaacacaccagccacgaaactctgtcttaatagagatcaagcgttcgca
V R D E N T P A T K L C L N R D Q A F A 390
1201 acagagcagctcagctgaagagtgcatgattatagaattaaagcatattctaaaaatgca
T E Q L S * 395
1281 tttaagtgtgcattgagactcaaagctgcagcgtgatgaggttacactgcctccaagt
Fig. S3. Nucleotide and deduced amino acid sequences of the zebrafish tac3ra (A) and tac3rb (B). Numbering of the deduced amino acid sequences begins
with the first methionine of the ORF to the right of each line. Nucleotide numbers are to the left of each line. The start and stop codons are shaded in gray.
These sequences have been deposited in the GenBank nucleotide database under accession numbers JF317292 and JF317293, respectively. Open circles, putative
N-glycosylation sites; open squares, putative protein kinase C phosphorylation sites; open triangle, putative cAMP and cGMP-dependent protein kinase
phosphorylation site; open diamonds, putative Casein kinase II phosphorylation sites; open trapezoid, putative tyrosine kinase phosphorylation site; open
octagons, putative N-myristoylation sites. Predicted transmembrane domains (TM1–TM7) are underlined; arrowheads indicate the exon-intron boundaries.
Biran et al. www.pnas.org/cgi/content/short/1119165109 5of9
TM3
TM4
TM6
TM2
TM1
TM5
30
70
110
150
190
250
290