In vitro anaerobic trinitrotoluene (TNT) degradation with rumen fluid ...

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Doctor of Philosophy thesis of Taejin Lee presented on November 30, 1994 APPROVED: Redacted for Privacy Major Professor, refresenting Civil Engineering Redacted for Privacy Chair of Department of Civil Engineering Dean of Graduate Sc Redacted for Privacy I understand that my thesis will become part of the permanent collection of Oregon State University libraries. My signature below authorizes release of my thesis to any reader upon request. Redacted for Privacy Taejin Lee, Author
ACKNOWLEDGMENTS I wish to express special appreciation to my major professor, Dr. Kenneth J. Willamson, for his advice, support and encouragement. He supervised the research work presented herein and provided professional suggestions for this study and was a most important contributor for this study as consultants, supervisor, and editor. I would also like to especially thank to Dr. A. Morrie Craig of the department of Veterinary Medicine for his invaluable direction and guidance during my project. Thanks are given also to my graduate committee members, Dr. Sandra L. Woods and Dr. Lewis Semprini <strong>with</strong> special thanks to Joseph Lotrario, who helped me <strong>with</strong> experiment preparation and guidance.
Page 1 and 2: AN ABSTRACT OF THE THESIS OF Taejin
Page 3: In Vitro Anaerobic Trinitrotoluene(
Page 7 and 8: 4.1 Abstract 4.2 Introduction 4.2.1
Page 9 and 10: LIST OF FIGURES Figure Page 1.1 Int
Page 11 and 12: Table LIST OF TABLES Page 2.1 Incub
Page 13 and 14: PREFACE This article is a compilati
Page 15 and 16: iological remediation of explosives
Page 17 and 18: was suspected that the azoxy compou
Page 19 and 20: ioremediation approach, subject to
Page 21 and 22: 10,14,16 02N 02 16,28 NO2 10,14,16
Page 23 and 24: Boopathy and Kulpa (3,4) used a sul
Page 25 and 26: 02N 02N 02N NO2 02 114,5,13,24 CH3
Page 27 and 28: 1.5 Discussion Based upon aerobic c
Page 29 and 30: 1.7 References 1. Aust, S.D. 1990.
Page 31 and 32: 23. Pres lan, J. E., B. B. Hatrel,
Page 33 and 34: the nitrogen source (2,5), and hydr
Page 35 and 36: 2.3.3 Analysis of the experiment Th
Page 37 and 38: 1 1 0 5 10 15 20 25 30 35 40 45 50
Page 39 and 40: ile: >T11-ft 20. 0 500 firti AA' K.
Page 41 and 42: The major peaks of the GC/MS chroma
Page 43 and 44: 2.6 References 1. Boopathy, R. and
Page 45 and 46: 20. Williams, R. T., P. S. Ziegenfu
Page 47 and 48: observed (2, 3, 6, 11). Recently, r
Page 49 and 50: Table 3.1 Sample conditions for exa
Page 51 and 52: 3.6 References 1. Bartell R. 1989.
Page 53 and 54: Chapter 4. Trinitrotoluene (TNT) Tr
Page 55 and 56:
degradation of TNT. Aerobic bacteri
Page 57 and 58:
4.2.2 Anaerobic processes Recently
Page 59 and 60:
Recently, researchers have studied
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Ethanol was used to prevent contami
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supernatant was anaerobically trans
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4.3.5 G.8 growth experiments with v
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E C 0.22 0.174 .0.128 9 C ° 0.082
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GRAPH A 24 12 20 12 ro 2 8 4 0 0 20
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4.5.1 TNT transformation G.8 was in
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4.5.3 26DNT transformation In the s
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BOTTLE A BOTTLE B BOTTLE C W.e'7131
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:MO 1,404 24404 00 :1044 14444 13a4
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BOTTLE A BOTTLE B BOTTLE C 66 44446
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4.5.6 Time course TNT transformatio
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Symbols: 0.020 0.015 0 1 2 3 4 5 6
Page 85 and 86:
4.7 References 1. 2. 7 Bartell R. 1
Page 87 and 88:
23. Montemagno, C. D. and R. L. Irv
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Chapter 5. Feasibility of Trinitrot
Page 91 and 92:
microorganism in full-scale bioreme
Page 93 and 94:
Gas chromatography/mass spectrometr
Page 95 and 96:
with identical physical properties
Page 97 and 98:
Figure 5.1 Schematic diagram of fix
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various times. Figure 6 also shows
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1 1 1.2 - 1.0 - 0.8 - 0.6 - 0.4 - 0
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9 8 7 rn 6 z 2 5 0 z a) 2 3 0 2 1 0
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The column experiment showed that c
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70 60 2 50 E o40 .16 700 C 0 020 10
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11. Fernando, T., J. A. Bumpus, and
Page 111 and 112:
Chapter 6. Conclusions TNT transfor
Page 113 and 114:
7 Bibliography 1. Aust, S.D. 1990.
Page 115 and 116:
23. Klausmeier, R. E., J. L. Osmon,
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44. Wachenheim, D. E., L.L. Blythe,
Page 119 and 120:
I.1. General I. Isolation of A Rume
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EM.1 - ct I... IA MIS EM -2 TM se -
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Table 11.1. TNT Derivatives Retenti
Page 125 and 126:
11.3 GC/MS Analysis for TNT and Int
Page 127 and 128:
Ion Chromatography for Anions Analy
Page 129 and 130:
III. TNT Extraction Methods and Med
Page 131 and 132:
111.2. Minimal Medium Preparation t
Page 133 and 134:
III.3. Plug Reactor Medium Preparat
Page 135:
02N NO2 02N NO2 H2 CH3 NO2 NO2 2,4,
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