Rainer Heintzmann

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STED, selecting a dye 16 S 2 Singlet S 1 ~P s0σI ex S 0 ps ps ~µs ∼P s1 τ –1 ~ I ex Triplet T 1 T 0 Rainer Heintzmann, 2007
17 STED Microscopy STED beam Resulting PSF 1 µm NA 1.3, 10nm pixelsize, no background Rainer Heintzmann, 2007
Page 1 and 2: Hochaufl Hochauflösende Hochaufl H
Page 3 and 4: 3 Bigger NA: 4Pi Microscopy Rainer
Page 5 and 6: 5 APD1 Leica 4Pi APD2 http://www.le
Page 7 and 8: 7 4Pi images Deviding Escherichia C
Page 9 and 10: 9 Aperture increase (3): ( ): I5 Mi
Page 11 and 12: 11 Stimulated Stimulated Emission m
Page 13 and 14: Saturation of the stimulated emissi
Page 15: STED, the details 15 Intensity Exci
Page 19 and 20: 19 4Pi-STED 4Pi STED images Fluores
Page 21 and 22: 21 STED Images Confocal STED human
Page 23 and 24: 23 Computational spectacles Methods
Page 25 and 26: Paradigm: Optimize for information
Page 27 and 28: 27 Overview �� Pointilism: Loca
Page 29 and 30: 29 Localization not resolution Loca
Page 31 and 32: 31 Can we separate Quantum Dots by
Page 33 and 34: 33 Close Separation 10 frames of th
Page 35 and 36: Outlook •Process entire images (m
Page 37 and 38: 37 Photo Activated Localization Mic
Page 39 and 40: 39 Problem: Limited Numerical umeri
Page 41 and 42: 41 OTF in Axial Tomography Aperture
Page 43 and 44: 43 Reconstructed Image Cluster of q
Page 45 and 46: 45 Biological Specimen Polytrichum
Page 47 and 48: Selective elective Plane lane Illum
Page 49 and 50: Selective elective Plane lane Illum
Page 51 and 52: Electrodes 51 Results (Cell Suspens
Page 53 and 54: 53 Linear Structured Illumination R
Page 55 and 56: 55 Patterned Excitation 2D Setup li
Page 57 and 58: 57 Convolution Rainer Heintzmann, 2
Page 59 and 60: 59 Convolution Rainer Heintzmann, 2
Page 61 and 62: 61 Separating the orders… Rainer
Page 63 and 64: 63 Separating the orders… Shiftin
Page 65 and 66: 65 Sample Widefield Image Reconstru
Page 67 and 68:
Structured Illumination experiments
Page 69 and 70:
Page 71 and 72:
Page 73 and 74:
Page 75 and 76:
75 Overview �� Pointilism: Loca
Page 77 and 78:
77 Non-linear Non linear Structured
Page 79 and 80:
R. Heintzmann, T.M. Jovin, and C. C
Page 81 and 82:
81 2D Patterned SPEM Approach Line
Page 83 and 84:
Saturation of Photoswitching as non
Page 85 and 86:
85 Intensity Photoswitchable Protei
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87 Conclusion: Computational specta
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Rainer Heintzmann

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