Rainer Heintzmann

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30 How to separate particles? Spectral precision distance microscopy Problems: Chromatic Aberrations, few dyes P. Edelmann, A. Esa, H. Bornfleth, R..Heintzmann, M. Hausmann, and C. Cremer. Proc. of SPIE , 3568:89-95, 1999 Using fluorescence lifetime for separation (FLIM) Problems: Lifetime depends on microenvironm. M. Heilemann, D.P. Herten, R.Heintzmann, C. Cremer, C. Müller, P. Tinnefeld, K.D. Weston, J. Wolfrum and M. Sauer. Anal. Chem., 74, 3511-3517, 2002. Can we use the blinking characteristics? Rainer Heintzmann, 2007
31 Can we separate Quantum Dots by their individual blinking? �� Localization >10 times better than resolution �� Quantum dots blink slow enough for imaging �� QDs blink independent �� Can we separate, even with strong overlap? 655 nm quantum dots on a cover slip K.A. Lidke, B. Rieger, T.M. Jovin, R. Heintzmann Optics Express 13, 7052-7062, 2005. Rainer Heintzmann, 2007
Page 1 and 2: Hochaufl Hochauflösende Hochaufl H
Page 3 and 4: 3 Bigger NA: 4Pi Microscopy Rainer
Page 5 and 6: 5 APD1 Leica 4Pi APD2 http://www.le
Page 7 and 8: 7 4Pi images Deviding Escherichia C
Page 9 and 10: 9 Aperture increase (3): ( ): I5 Mi
Page 11 and 12: 11 Stimulated Stimulated Emission m
Page 13 and 14: Saturation of the stimulated emissi
Page 15 and 16: STED, the details 15 Intensity Exci
Page 17 and 18: 17 STED Microscopy STED beam Result
Page 19 and 20: 19 4Pi-STED 4Pi STED images Fluores
Page 21 and 22: 21 STED Images Confocal STED human
Page 23 and 24: 23 Computational spectacles Methods
Page 25 and 26: Paradigm: Optimize for information
Page 27 and 28: 27 Overview �� Pointilism: Loca
Page 29: 29 Localization not resolution Loca
Page 33 and 34: 33 Close Separation 10 frames of th
Page 35 and 36: Outlook •Process entire images (m
Page 37 and 38: 37 Photo Activated Localization Mic
Page 39 and 40: 39 Problem: Limited Numerical umeri
Page 41 and 42: 41 OTF in Axial Tomography Aperture
Page 43 and 44: 43 Reconstructed Image Cluster of q
Page 45 and 46: 45 Biological Specimen Polytrichum
Page 47 and 48: Selective elective Plane lane Illum
Page 49 and 50: Selective elective Plane lane Illum
Page 51 and 52: Electrodes 51 Results (Cell Suspens
Page 53 and 54: 53 Linear Structured Illumination R
Page 55 and 56: 55 Patterned Excitation 2D Setup li
Page 57 and 58: 57 Convolution Rainer Heintzmann, 2
Page 59 and 60: 59 Convolution Rainer Heintzmann, 2
Page 61 and 62: 61 Separating the orders… Rainer
Page 63 and 64: 63 Separating the orders… Shiftin
Page 65 and 66: 65 Sample Widefield Image Reconstru
Page 67 and 68: Structured Illumination experiments
Page 75 and 76: 75 Overview �� Pointilism: Loca
Page 77 and 78: 77 Non-linear Non linear Structured
Page 79 and 80: R. Heintzmann, T.M. Jovin, and C. C
Page 81 and 82:
81 2D Patterned SPEM Approach Line
Page 83 and 84:
Saturation of Photoswitching as non
Page 85 and 86:
85 Intensity Photoswitchable Protei
Page 87 and 88:
87 Conclusion: Computational specta
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Rainer Heintzmann

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