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Rainer Heintzmann

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39<br />

Problem: Limited Numerical<br />

umerical Aperture perture<br />

z<br />

x,y<br />

Cell<br />

Objective Lense<br />

Immersion Medium<br />

Cover Slip<br />

Slide<br />

α<br />

Immersion<br />

Medium<br />

NA = n sin α<br />

<strong>Rainer</strong> <strong>Heintzmann</strong>

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