Rainer Heintzmann
Rainer Heintzmann
Rainer Heintzmann
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9<br />
Aperture increase (3): ( ):<br />
I5 Microscope<br />
icroscope<br />
incoherent<br />
ncoherent illumination<br />
llumination interference<br />
nterference<br />
imag mage interference<br />
nterference<br />
Sample<br />
CCD<br />
Camera<br />
z<br />
Dichroic Mirror<br />
White Light<br />
Source<br />
Offset!!<br />
Fluorescence Emisssion<br />
• No XY scanning required<br />
(full field illum., full field det.)<br />
• No laser required<br />
• Higher background<br />
no pinhole ⇒ more signal<br />
Fluorescence<br />
Intensity<br />
z<br />
<strong>Rainer</strong> <strong>Heintzmann</strong>, 2007
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