micropropagation studies in dracaena and cordyline - ETD ...

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Dracaena Cordyline Plate.1. Plant materials used for the study
3.2.2 Quality of Chemicals All chemicals used in the experiments were of analytical grade. 3.3 Preparation of culture media The stock solutions were mixed in the required proportion along with growth regulators and sucrose (3%). Then the volume was adjusted by adding double distilled water. The pH of the medium was adjusted 5.6 to 5.8 using digital pH meter by adding either 0.1N HCl or NaOH drop wise while stirring with the help magnetic stirrer. Volume was finally made up to required level and agar (0.7%) was added to the medium. Agar in the medium was made to melt by gentle heating and the medium was poured into the baby jar bottles having 300 ml capacity. 3.4 Sterilization procedures 3.4.1 Media Baby jars or culture tubes containing the media were autoclaved at a temperature of 121ºC at 15 lb per square inch pressure for 20 minutes. 3.4.2 Instruments All the instruments used for sterile handling and transfer of cultures were sterilized by autoclaving at a temperature of 121ºC at 15 lb per square inch pressure for 20 minutes. 3.4.3 Sterile technique in laminar air flow cabinets All the sterile transfer work was carried out in a laminar air flow cabinet. Before starting any sterile operation, the inner surface of the cabinet was swabbed with 70% ethyl alcohol. The UV lamp provided within the cabinet was then switched on for 15 to 20 minutes before use, followed by the airflow. During the course of transfer, between each transfer of explants to culture bottles or tubes, the instruments were dipped in alcohol and flame sterilized. 3.5 Culture establishments 3.5.1 Inoculation Before inoculation of explants, the ultra violet light of laminar air flow was switched on for 20 minutes for sterilization of the area. Inoculation was carried out under aseptic condition under laminar air flow. 3.5.2 Culture condition The culture tube or jams bottles were incubated in culture room having control over temperature and light. The temperature of culture room was maintained at 25 ± 2ºC with light intensity of 16 hours light and 8 hours dark. 3.5.3 Subculture Microshoots formed in the bottles were taken out and were separated by dissecting them in the sterile environment of laminar airflow cabinet with sterile dissecting needle/scalpel and forceps. Then they were placed back in the test tubes/ bottles containing the fresh media.
Page 1 and 2: MICROPROPAGATION STUDIES IN DRACAEN
Page 3 and 4: CONTENTS Sl. No. Chapter Particular
Page 5 and 6: LIST OF PLATES Plate No. TITLE 1. P
Page 7 and 8: optimize the micro propagation prot
Page 9 and 10: also likely to influence the uptake
Page 11 and 12: Vinterhalter (1989) showed that the
Page 13: 3. MATERIAL AND METHODS The present
Page 17 and 18: c. Per cent response of explants: T
Page 19 and 20: Replication : 4 Number of tubes per
Page 21 and 22: 4. EXPERIMENTAL RESULTS The results
Page 23 and 24: Table 1b: Effect of surface sterili
Page 25 and 26: Table 2: Effect of explants types o
Page 27 and 28: Table 3.1: Effect of BAP on number
Page 29 and 30: a) 8 mg/I BAP b) 10 mg/I BAP Plate.
Page 31 and 32: Plate.6. Shoot multiplication of co
Page 33 and 34: Table 5: Effect of GA3 on number of
Page 35 and 36: treated shoots produced significant
Page 37 and 38: Table 6: Effect of different IBA co
Page 39 and 40: 5. DISCUSSION The present investiga
Page 41 and 42: demonstrated that the highest shoot
Page 43 and 44: 6. SUMMARY The present study on mic
Page 45 and 46: Kwapata, K., Sabzikar, R., Sticklen
Page 47 and 48: APPENDIX Appendix I: Composition of

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