micropropagation studies in dracaena and cordyline - ETD ...

3.5.4 Rooting
The micro shoots were taken out and placed back in the tubes containing media with
different concentrations of IBA and NAA for rooting.
3.5.5 Ex vitro rooting and Hardening
Microshoots were taken out from the testtube, base of the shoot was moistened,
treated with powder form IBA of different concentrations and planted in small pots containing
different hardening media. These plants were maintained in polyhouse. They were watered
once in two days initially, then once in a week. Later they were transferred to green house
after 15 days for further acclimatization.
3.6 Experimental details
3.6.1 Experiment 1 - Standardization of surface sterilization
In this experiment, the efficacy of HgCl 2 with a treatment of a fungicide and a
bactericide for disinfecting the explants was studied. Fungicide (carbendazim) and bactericide
(streptomycin) were used for pretreatment in four treatments. Experiment was undertaken for
dracaena and cordyline.
3.6.1.1 a Treatment details
T1 - 0.05% HgCl2 for 5 minutes
T2 - 0.1 % HgCl2 for 5 minutes
T3 - 0.05% HgCl2 for 10 minutes
T 4 - 0.1% HgCl 2 for 10 minutes
T5 - 0.05% HgCl2 for 5 minutes and explants pretreated with fungicide and
bactericide
T6 - 0.1 % HgCl2 for 5 minutes and explants pretreated with fungicide and bactericide
T 7 - 0.05% HgCl 2 for 10 minutes and explants pretreated with fungicide and
bactericide
T8 - 0.1% HgCl2 for 10 minutes and explants pretreated with fungicide and
bactericide
T 9 – Control (without any chemicals and pretreatment)
Design : CRD
Replication : 3
Number of tubes per treatment :10
3.6.1.1 b Observations recorded
a. Percent contamination: Out of total tubes inoculated, the Number of tubes contaminated
were counted and expressed in percentage.
b. Per cent survival of explants: The number of plants survived out of total inoculated was
counted and converted in to percentage.