micropropagation studies in dracaena and cordyline - ETD ...

c. Per cent response of explants: The number of plants responded out of survived was
counted and converted in to percentage.
3.6.2 Experiment 2 – Standardizing explants for culture establishments
Different explants were used to study the response of different parts for shoot
generation and multiplication for both the species dracaena and cordyline.
3.6.2.1a Treatment details
T1 - Shoot tip
T2 - Nodal cuttings
T 3 - Leaf base
Design : CRD
Replication : 7
Number of tubes per treatment : 10
3.6.2.1b Observations recorded
a. Number of days taken for initiation of shoot: Number of days taken to show initial
differentiation of shoot from the date of inoculation of different explants recorded and
were expressed in mean number of days.
b. Percentage response: The number of plants responded out of inoculated was
counted and converted in to percentage.
c. Number of shoots per explants: The number of shoots produced from single
explants were noted.
3.6.3 Experiment 3 - Standardising different growth regulators and their
concentrations for shoot multiplication and rooting
The effect of growth regulator’s combinations on shoot proliferation and rooting from
explants cultured on MS medium fortified with different levels of cyokinins and combinations
with auxins were studied. First the effect of different concentrations of BAP on shoot
multiplication studied, followed by BAP in combination with different concentrations of NAA.
As the shoots formed were relatively shorter the effect of different concentrations of GA3 was
also evaluated to improve shoot length. Two auxins namely IBA and NAA were supplemented
to the culture medium to study the effect of the same in rooting. As the dracaena didn’t
respond to the above experiments further studies were continued only with cordyline.
(I) Influence of BAP on shoot multiplication
3.6. 3. I a Treatment details
T1 - 0.25 mg/l
T2 - 0.5 mg/l
T3 - 1.0 mg/l
T4 - 2.0 mg/l
T5 - 4.0 mg/l