2011 Jornada Científica de la Sociedad Española de Arcillas 14 de ...

Jornada Científica de la Sociedad Española de Arcillas. 14 de Noviembre de 2011
LIPID-BASED BIO-NANOHYBRIDS FOR THERMAL STABILIZATION OF
VIRAL SPECIES AND APPLICATION AS INFLUENZA VACCINES
B. Wicklein*, M.A. Martin del Burgo**, M. Yuste**, M. Darder*, P. Aranda*, J.
Ortin***, G. del Real**, E. Ruiz-Hitzky*
*Instituto de Ciencia de Materiales de Madrid, CSIC. Cantoblanco, 28049
Madrid
**Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Ctra.
de la Coruña Km 7,5, 28040 Madrid
***Centro Nacional de Biotecnología, CSIC, Cantoblanco, 28049 Madrid
We report the preparation and efficacy testing of influenza A vaccines
composed of bio-nanohybrids based on phospholipid modified clays [1] as
carrier for either inactivated influenza A virus particles (PR8/H1N1) or the main
surface influenza antigen hemagglutinin (HA) (Fig. 1A and B) [2]. Thermal
stabilization of vaccines during shipment and distribution is a challenging task,
especially in developing countries. To address this question, we have designed
a novel carrier platform based on two types of clays, sepiolite and Mg/Al type
layered double hydroxide, displaying biomimetic lipid interfaces to ensure native
and maximum stability of immobilized antigens. It is well known that solid
adjuvants as vaccine carriers may inflict conformational alterations on the
antigen by strong interactions and thus, diminish its immunogenic potential [3].
Therefore, lipid bilayer membranes are incorporated to modulate these
interactions. In addition to the use of the whole viral particle, the surface antigen
HA was also immobilized on the lipid interface mimicking its localization on the
viral membrane. As a result, these vaccine modifications preserved the
biological nature of the antigens (Fig. 1C), elicited virus-specific antibodies in
mice and showed higher thermal stability as compared to aluminum salts, the
currently most widely used antigenic adjuvant [4].
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