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LA TECNOLOGIA del DNA RICOMBINANTE

LA TECNOLOGIA del DNA RICOMBINANTE

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Cosmidi<br />

Possono trasportare 40 kb di <strong>DNA</strong> clonato e possono essere mantenuti come plasmidi in E. Coli.<br />

Vector system Host cell Insert capacity (kb)<br />

Plasmid E. coli 0.1-10<br />

Bacteriophage l E. coli 10-20<br />

Cosmid E. coli 35-45<br />

Bacteriophage P1 E. coli 80-100<br />

BAC (bacterial artificial<br />

chromosome)<br />

P1 bacteriophage-derived<br />

AC<br />

Vettori di clonaggio<br />

E. coli 50-300<br />

E. coli 100-300<br />

YAC Yeast 100-2,000

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