LA TECNOLOGIA del DNA RICOMBINANTE

Hybridisation
• I due strand di DNA sono complementari l’uno all’altro.
• The mRNA synthesized from a gene is identical to one strand of the
DNA and complementary to the other.
• Complementary strands could be separated (denaturation) and when
allowed to rejoin (renaturation) do so with high degree of specificity
forming DNA-DNA, DNA-RNA, or RNA-RNA hybrids.
Methods developed on these properties:
• Southern blot: Hybridization of DNA to DNA (solid/solution).
• Northern blot: Hybridization of DNA to RNA (solid/solution).
• RNAse protection assay: Hybridization of RNA to RNA (solution).
• In situ hybridisation: DNA-DNA or DNA-RNA (tissue section, cells)