MICOBATTERI - Sezione di Microbiologia
MICOBATTERI - Sezione di Microbiologia
MICOBATTERI - Sezione di Microbiologia
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NaOH-<br />
NALC<br />
Vortex and<br />
stand for 15<br />
minutes<br />
PBS<br />
Fill tube<br />
to 14ml<br />
Centrifuge<br />
15 min 3000g<br />
7H9<br />
PANTA<br />
OADC<br />
sample 1 sample 2 Negative QC sample 3 sample 4 sample 5 or<br />
Positive QC<br />
Sputum decontamination<br />
900µl / well<br />
C<br />
C C<br />
C C<br />
1 3 4<br />
C<br />
MODS<br />
1 2 NEG 3 4 5 / POS<br />
control<br />
control<br />
C<br />
C<br />
C<br />
C<br />
C<br />
C<br />
Culture and<br />
<strong>di</strong>rect<br />
susceptibility<br />
testing<br />
Drug-free<br />
Isoniazid<br />
Rifampici<br />
I<br />
R<br />
I<br />
R<br />
I<br />
R<br />
I<br />
R<br />
I<br />
R<br />
I<br />
R<br />
2 wells for drug-free control (C), 1 for isoniazid 0.4 µg/ml (I), 1 for rifampicin 1 µg/ml (R)<br />
Istituto <strong>di</strong> <strong>Microbiologia</strong> - Genova<br />
61<br />
62<br />
Moore DAJ et al. Microscopic-observation drug-susceptibility assay for the <strong>di</strong>agnosis of TB. New Eng J Med 355: 1539–1551, 2006<br />
63<br />
64<br />
16
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