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Agilent ZORBAX 液ç¸æ±éæ©æå - ä¸å½ç§å¦é¢ä¸æµ·ç»èçç©å¦ç 究æ
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Extend-C18 StableBond SB-C18 pH <br />
A <br />
B <br />
C <br />
<br />
<br />
<br />
<strong>ZORBAX</strong> SB-C18<br />
883975-902<br />
4.6 x 150 mm, 5 µm<br />
<strong>ZORBAX</strong> Extend-C18<br />
773450-902<br />
4.6 x 150 mm, 5 µm<br />
<strong>ZORBAX</strong> Rx-C18<br />
883967-902<br />
4.6 x 150 mm, 5 µm<br />
<br />
50% 50% + 1% TFA<br />
<br />
60% 40% <br />
<br />
1.5 mL/min<br />
<br />
90°C<br />
<br />
<br />
Fraction of Toluene k Remaining<br />
A<br />
B<br />
C<br />
Column Volumes Mobile Phase<br />
300Extend-C18 pH pH pH 2 pH 11.5<br />
300Extend-C18 pH <br />
pH pH <br />
<br />
Extend-C18 LC/MS <br />
<br />
<strong>ZORBAX</strong> Extend-C18<br />
773700-902<br />
2.1 x 150 mm, 5 µm<br />
A 0.1% TFA <br />
B0.085% TFA80%<br />
A10 mM NH 4 OH <br />
B10 mM NH 4 OH80% <br />
0.2 mL/min<br />
15 min 15-50% B<br />
35°C<br />
MS ESI - Vf 70V<br />
4.5 kV, N2- 35 psi, 12 L/min.325°C<br />
2.5 µL 50 pmol<br />
IIIIII<br />
B.E Boyes Separation and Analysis of Peptides at High pH<br />
Using RP-HPLC/ESI-MS, 4th WCBP, San Francisco, CA Jan 2000.<br />
TIC (200-1500 m/z)<br />
TIC (200-1500 m/z)<br />
min<br />
min<br />
pH pH pH<br />
<br />
pH <br />
Extend-C18 pH <br />
m/z<br />
m/z<br />
100<br />
www.agilent.com/chem/store
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