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HPLC 2-D HPLC : : : : : MS : ZORBAX 300SB-C18 5065-9913 0.3 x 5 mm, 5 µm ZORBAX 300SB-C18 5065-9911 0.075 x 150 mm, 3.5 µm : 3% /0.1% : A = , 0.1% , B = , 0.1% : 30 µL/min : 300 nL/min : : 6 min = 3%B, 120 min = 60%B, 125 min = 80%B, 130 min = 80% B, 131 min = 3%B, 140 min = 3% B : Nano ESI, : 5L/min, .: 225C. : : 1:35 V, : 115 V, 1:12 V, 2:3.5 V, : 80 V. ICC: on, : 4, : 150 ms; 60.000, , MS/MS : 1 - 8 µL Salt Step Elution: 8ml 10 mM-100 mM KCI (10 mM ), 125 mM, 150 mM, 200 mM, 300 mM, 500 mM, 1M. Time (min) 30 mM KCI 60 mM KCI 75 mM KCI 120 mM KCI (BSA) HPLC BSA 78 www.agilent.com/chem/store
ZORBAX Poroshell Eclipse 300SB- 300Extend Bio-SCX (mm) (µm) SB-C18 XDB-C18 C18 300SB-C8 300SB-C8 C18 II 0.8 x 50 3.5 5065-9942 0.5 x 250 5 5064-8258 5064-8286 5064-8266 0.5 x 150 5 5064-8256 5064-8287 5064-8264 0.5 x 75 5 5065-4468 0.5 x 35 5 5064-8254 5064-8296 5064-8294 * 0.5 x 35 3.5 5064-8260 5064-8298 5065-4459 0.3 x 250 5 5064-8257 5064-8269 5064-8265 0.3 x 150 5 5064-8255 5064-8291 5064-8263 0.3 x 35 5 5064-8253 5064-8297 5064-8295 0.3 x 35 3.5 5065-9912 * 0.3 x 150 3.5 5064-8261 5064-8271 5064-8267 5065-4460 5065-4464 * 0.3 x 100 3.5 5064-8259 5065-4461 5065-4465 * 0.3 x 75 3.5 5064-8270 5065-4462 5065-4466 * 0.3 x 50 3.5 5064-8300 5065-4463 5065-4467 10/ 5065-4427 5065-4427 5065-4427 5065-4427 5065-4427 5065-4427 *RR 3.5 µm 300SB-C18 (mm) (µm) USP L1 ** 0.1 x 150 3.5 5065-9910 ** 0.075 x 150 3.5 5065-9911 300SB-C8 USP L7 * 0.075 x 50 3.5 5065-9924 5065-9923 /5/ 0.3 x 5 5 5065-9913 5065-9914 / 5065-9915 5065-9915 *RR 3.5 µm www.agilent.com/chem/store 79
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Agilent ZORBAX
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Agilent 1200 20 1200 HPLC 40
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ZORBAX USP Agilemt Z
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ZORBAX ZORBAX ZORBAX RP-HPLC
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ZORBAX MW < 2000 MW > 2000 5µm
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ZORBAX C18 C8 C3CN C8C18 C8
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ZORBAX pH 1-12 pH pH pH pH
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ZORBAX pH pH Eclipse Plus
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ZORBAX pH 3 2a Eclipse XDB 6
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ZORBAX ZORBAX ZORBAX pH
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ZORBAX • • • pH •
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ZORBAX pH 2 pH 7 : 1. 2. 3.
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ZORBAX ZORBAX Eclipse Plus Eclips
Page 29 and 30: ZORBAX ZORBAX Eclipse XDB pH :
Page 31 and 32: ZORBAX Eclipse XDB A B C Ecli
Page 33 and 34: ZORBAX ZORBAX Eclipse XDB (mm) (
Page 35 and 36: ZORBAX ZORBAX 80Å StableBond pH
Page 37 and 38: ZORBAX RRHT mAU 450 400 350 300
Page 39 and 40: ZORBAX ZORBAX 80Å StableBond
Page 41 and 42: ZORBAX ZORBAX Rx Eclipse XDB-C18
Page 43 and 44: ZORBAX ZORBAX 80Å Extend-C18 pH
Page 45 and 46: ZORBAX Extend-C18 pH : ZORBAX Ex
Page 47 and 48: ZORBAX ZORBAX Bonus-RP pH pH
Page 49 and 50: ZORBAX pH pH ZORBAX Bonus-RP :
Page 51 and 52: ZORBAX ZORBAX pH 2-9 Eclipse
Page 53 and 54: ZORBAX ZORBAX ZORBAX A (mm)
Page 55 and 56: ZORBAX pH ZORBAX Rx-SIL 80
Page 57 and 58: ZORBAX (3.5 µm) (1.8 µm) Ecli
Page 59 and 60: ZORBAX 1.8 µm 1.8 µm [600 bar
Page 61 and 62: 20X : : : : ZORBAX SB-C18 883975-
Page 63 and 64: (1.8µm) (3.5µm) N (3.5 µm)*
Page 65 and 66: Zorbax 400 bar6000 psi XDB-C18
Page 67 and 68: : ZORBAX SB-C18 866953-902 4.6 x 75
Page 69 and 70: ZORBAX 3.5 µm RxBonus-RP Extend-
Page 71 and 72: A B : : ZORBAX SB-C18 863953-902
Page 73 and 74: ZORBAX 1.0 mm LC/MS 1 mm 2
Page 75 and 76: ZORBAX LC/MS 0.50.30.1 0.0
Page 77 and 78: : : : : ZORBAX SB-C18 5064-8255 0.
Page 79: LC/MS ZORBAX HPLC 1100/1200 1.
Page 83 and 84: ZORBAX PrepHT DAD Vial 1 | 347 V
Page 85 and 86: ZORBAX PrepHT Bonus-RP Extend-C18
Page 87 and 88: Agilent Prep C18 : Agilent Prep-C1
Page 89 and 90: Ultron Ultron ES-OVM ES-Pepsin
Page 91 and 92: ZORBAX HPLC HPLC Agilent ZORBAX
Page 93 and 94: ZORBAX pH 0.1% 300Å 30-60 m
Page 95 and 96: ZORBAX pH pH MW < 50 kDa 300SB-
Page 97 and 98: ZORBAX ZORBAX 300SB-C3 pH ZORBA
Page 99 and 100: ZORBAX ZORBAX 300Å StableBond
Page 101 and 102: ZORBAX ZORBAX 300Å Extend-C18 p
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Page 105 and 106: ZORBAX Poroshell ZORBAX Po
Page 107 and 108: ZORBAX ZORBAX Eclipse (AAA) 24
Page 109 and 110: ZORBAX ZORBAX GF-250 GF-450
Page 111 and 112: ZORBAX 9.4 x 250 mm ZORBAX GF-250
Page 113 and 114: ZORBAX ZORBAX SAX SCX ZORBAX S
Page 115 and 116: ZORBAX Eclipse Plus • • •
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