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Extend-C18 StableBond SB-C18 pH A B C ZORBAX SB-C18 883975-902 4.6 x 150 mm, 5 µm ZORBAX Extend-C18 773450-902 4.6 x 150 mm, 5 µm ZORBAX Rx-C18 883967-902 4.6 x 150 mm, 5 µm 50% 50% + 1% TFA 60% 40% 1.5 mL/min 90°C Fraction of Toluene k Remaining A B C Column Volumes Mobile Phase 300Extend-C18 pH pH pH 2 pH 11.5 300Extend-C18 pH pH pH Extend-C18 LC/MS ZORBAX Extend-C18 773700-902 2.1 x 150 mm, 5 µm A 0.1% TFA B0.085% TFA80% A10 mM NH 4 OH B10 mM NH 4 OH80% 0.2 mL/min 15 min 15-50% B 35°C MS ESI - Vf 70V 4.5 kV, N2- 35 psi, 12 L/min.325°C 2.5 µL 50 pmol IIIIII B.E Boyes Separation and Analysis of Peptides at High pH Using RP-HPLC/ESI-MS, 4th WCBP, San Francisco, CA Jan 2000. TIC (200-1500 m/z) TIC (200-1500 m/z) min min pH pH pH pH Extend-C18 pH m/z m/z 100 www.agilent.com/chem/store
ZORBAX ZORBAX 300Å Extend-C18 (mm) (µm) 4.6 x 250 5 770995-902 4.6 x 150 5 773995-902 4.6 x 150 3.5 763973-902 4.6 x 100 3.5 761973-902 4.6 x 50 3.5 765973-902 * 2.1 x 150 3.5 763750-902 * 2.1 x 100 3.5 761775-902 * 2.1 x 50 3.5 765750-902 4/ 4.6 x 12.5 5 820950-932 4/ 2.1 x 12.5 5 821125-932 820888-901 *RR 3.5 µm * 0.3 x 150 3.5 5065-4464 * 0.3 x 100 3.5 5065-4465 * 0.3 x 75 3.5 5065-4466 * 0.3 x 50 3.5 5065-4467 www.agilent.com/chem/store 101
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Agilent ZORBAX
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Agilent 1200 20 1200 HPLC 40
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ZORBAX USP Agilemt Z
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ZORBAX ZORBAX ZORBAX RP-HPLC
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ZORBAX MW < 2000 MW > 2000 5µm
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ZORBAX C18 C8 C3CN C8C18 C8
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ZORBAX pH 1-12 pH pH pH pH
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ZORBAX pH pH Eclipse Plus
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ZORBAX pH 3 2a Eclipse XDB 6
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ZORBAX ZORBAX ZORBAX pH
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ZORBAX • • • pH •
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ZORBAX pH 2 pH 7 : 1. 2. 3.
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ZORBAX ZORBAX Eclipse Plus Eclips
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ZORBAX ZORBAX Eclipse XDB pH :
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ZORBAX Eclipse XDB A B C Ecli
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ZORBAX ZORBAX Eclipse XDB (mm) (
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ZORBAX ZORBAX 80Å StableBond pH
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ZORBAX RRHT mAU 450 400 350 300
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ZORBAX ZORBAX 80Å StableBond
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ZORBAX ZORBAX Rx Eclipse XDB-C18
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ZORBAX ZORBAX 80Å Extend-C18 pH
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ZORBAX Extend-C18 pH : ZORBAX Ex
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ZORBAX ZORBAX Bonus-RP pH pH
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ZORBAX pH pH ZORBAX Bonus-RP :
Page 51 and 52: ZORBAX ZORBAX pH 2-9 Eclipse
Page 53 and 54: ZORBAX ZORBAX ZORBAX A (mm)
Page 55 and 56: ZORBAX pH ZORBAX Rx-SIL 80
Page 57 and 58: ZORBAX (3.5 µm) (1.8 µm) Ecli
Page 59 and 60: ZORBAX 1.8 µm 1.8 µm [600 bar
Page 61 and 62: 20X : : : : ZORBAX SB-C18 883975-
Page 63 and 64: (1.8µm) (3.5µm) N (3.5 µm)*
Page 65 and 66: Zorbax 400 bar6000 psi XDB-C18
Page 67 and 68: : ZORBAX SB-C18 866953-902 4.6 x 75
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Page 71 and 72: A B : : ZORBAX SB-C18 863953-902
Page 73 and 74: ZORBAX 1.0 mm LC/MS 1 mm 2
Page 75 and 76: ZORBAX LC/MS 0.50.30.1 0.0
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Page 79 and 80: LC/MS ZORBAX HPLC 1100/1200 1.
Page 81 and 82: ZORBAX Poroshell Eclipse 300SB-
Page 83 and 84: ZORBAX PrepHT DAD Vial 1 | 347 V
Page 85 and 86: ZORBAX PrepHT Bonus-RP Extend-C18
Page 87 and 88: Agilent Prep C18 : Agilent Prep-C1
Page 89 and 90: Ultron Ultron ES-OVM ES-Pepsin
Page 91 and 92: ZORBAX HPLC HPLC Agilent ZORBAX
Page 93 and 94: ZORBAX pH 0.1% 300Å 30-60 m
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Page 105 and 106: ZORBAX Poroshell ZORBAX Po
Page 107 and 108: ZORBAX ZORBAX Eclipse (AAA) 24
Page 109 and 110: ZORBAX ZORBAX GF-250 GF-450
Page 111 and 112: ZORBAX 9.4 x 250 mm ZORBAX GF-250
Page 113 and 114: ZORBAX ZORBAX SAX SCX ZORBAX S
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