Agilent ZORBAX 液相柱选择指南 - ä¸­å›½ç§‘å­¦é™¢ä¸Šæµ·ç»†èƒžç”Ÿç‰©å­¦ç ”ç©¶æ‰€

HPLC 2-D HPLC
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MS :
ZORBAX 300SB-C18
5065-9913
0.3 x 5 mm, 5 µm
ZORBAX 300SB-C18
5065-9911
0.075 x 150 mm, 3.5 µm
: 3% /0.1%
: A = , 0.1% , B = , 0.1%
: 30 µL/min
: 300 nL/min
:
:
6 min = 3%B, 120 min = 60%B,
125 min = 80%B,
130 min = 80% B, 131 min = 3%B,
140 min = 3% B
: Nano ESI, : 5L/min,
.: 225C.
: : 1:35 V, :
115 V, 1:12 V,
2:3.5 V, : 80 V. ICC: on,
: 4, : 150 ms;
60.000, , MS/MS
: 1 - 8 µL
Salt Step Elution: 8ml 10 mM-100 mM
KCI (10 mM ), 125 mM,
150 mM, 200 mM, 300 mM, 500 mM, 1M.
Time (min)
30 mM KCI
60 mM KCI
75 mM KCI
120 mM KCI
(BSA) HPLC
BSA
78
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