Agilent ZORBAX 液ç¸æ±éæ©æå - ä¸å½ç§å¦é¢ä¸æµ·ç»èçç©å¦ç 究æ
Agilent ZORBAX 液ç¸æ±éæ©æå - ä¸å½ç§å¦é¢ä¸æµ·ç»èçç©å¦ç 究æ
Agilent ZORBAX 液ç¸æ±éæ©æå - ä¸å½ç§å¦é¢ä¸æµ·ç»èçç©å¦ç 究æ
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HPLC UV MS <br />
:<br />
<strong>ZORBAX</strong> 300SB-C18<br />
5064-8263<br />
0.3 x 150 mm, 5 µm<br />
UV<br />
:<br />
:<br />
:<br />
MS :<br />
<br />
50 min 5-55% B 55-57 min 85% B<br />
A 0.1% <br />
B 0.1% <br />
5.5 µL/min<br />
206 nm<br />
LC/MS:ESI <br />
LC/MSD -Vcap 4000 V<br />
7 L/min<br />
250°C<br />
15 psi<br />
50 V<br />
300 ms<br />
3<br />
3 m/z<br />
1.0 V<br />
100 nL<br />
(4 pmol)<br />
UV at 206 nm<br />
% Relative Abundance<br />
MS<br />
Time (min)<br />
<strong>ZORBAX</strong> 300SB-C18 0.3 mm<br />
UV MS MS <br />
Time (min)<br />
LC/MS <strong>ZORBAX</strong> <br />
:<br />
<strong>ZORBAX</strong> 300SB-C18<br />
5065-9911<br />
0.075 x 150 mm, 3.5 µm<br />
Intens.<br />
:<br />
:<br />
:<br />
:<br />
<br />
A + 0.1% <br />
B+ 0.1% <br />
600 nl/min<br />
25 min 2% B 52% B<br />
MS <br />
100 fm (1 µl) 8 <br />
<strong>ZORBAX</strong> Nano HPLC 0.075 mm<br />
LC/MS <br />
Time (min)<br />
96<br />
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