Applicazioni Real-Time PCR - Bgbunict.it

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Creating an Allelic Discrimination (AD) Plate Document An AD plate document stores data collected from an AD run for a single reaction plate. An AD plate document also stores other information about the run, including sample names, markers, and detectors.
AD plate documents use: • Detector – In SDS software, a virtual representation of a TaqMan® probe and primer set and associated fluorescent dye that detects a single target nucleic acid sequence. • Markers – A set of two detectors that discriminate between different alleles of a common locus. Allele 1 is detected by one detector (for example, FAM dye), and allele 2 is detected by the second detector (for example, VIC® dye). • Task – A setting that you apply to the markers in a well of a plate document and that determines the way the SDS software uses the data collected from the well during analysis.
Page 1 and 2: Metodi e tecniche di laboratorio II
Page 3 and 4: Results Transcriptomics “at a gla
Page 5 and 6: Spesso nei carcinomi umani vengono
Page 7 and 8: Il kit K-RAS è in grado di identif
Page 9 and 10: La ARMS (Amplification Refractory M
Page 11 and 12: Analisi dei dati: metodo ∆Ct Nei
Page 13 and 14: Ogni saggio di mutazione, marcato c
Page 15 and 16: Aggiungere immediatamente 5 µl di
Page 17 and 18: ABI 7900
Page 19: Ct del saggio di controllo tra 29 e
Page 22 and 23: 3. Nella scheda ‘Amplification Pl
Page 24 and 25: 2. Valori ∆Ct della miscela stand
Page 26 and 27: ABI7900
Page 28 and 29: The actual quantity of target seque
Page 31 and 32: About AD Experiments AD Experiment
Page 33 and 34: Quality of DNA Ensure that the DNA
Page 35: Preparing the Plate 1. Invert the r
Page 39 and 40: 1) Performing the Pre-Read Run 2) P
Page 41 and 42: Evaluating Results After identifyin

Applicazioni Real-Time PCR - Bgbunict.it

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