Karen Amaral Tavares Pinheiro - Universidade Católica de Pelotas

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124 underwent prenatal follow-up, and the inclusion criteria included the following specifications: more than 18 years old, living in the urban area, not taking antidepressants or mood regulators and capable of understanding and completing the neuropsychiatric questionnaires in the postpartum period. Between 60 to 90 days after delivery, the women were re-interviewed at home and provided blood for NGF, BDNF, and IL-6 determination and saliva for cortisol analyses. Cortisol was measured using salivary levels in both mothers and infants. In the 4 th month of life, infants were evaluated for neurobiological - motor development. All women answered a confidential self-administered questionnaire that included questions about socioeconomic conditions, age, previous psychiatric treatment, use of psychoactive drugs in the past, previous suicide attempts, smoking habits, alcohol consumption, living with the father and the type of delivery. PPADs (depression, hypomanic/manic and mixed episode) and anxiety disorders were assessed using the Mini International Neuropsychiatric Interview (MINI Portuguese - version 5.0 Plus) (29). MINI is a short structured interview that is compatible with DSM-IV criteria. It establishes a dichotomic variable for the presence or absence of PPAD. Stressful life events (SLEs) were assessed by means of six questions obtained from life events and coping scales (30). The women were questioned in the postpartum period about the following issues during the gestational period: the death of someone in the family, grave sickness, a change of address, unemployment, disengagement and the risk of abortion. The Alberta Infant Motor Scale (AIMS) was used to evaluate the infants’ neurobiological -motor development (31). The AIMS, which is an observational assessment scale, is designed to measure gross motor maturation in infants from birth through independent walking (18 months). Based on the literature, 58 items were generated and organized into four positions: prone, supine, sitting and standing. Each item describes three aspects of motor performance: weight-bearing, posture and antigravity movements. Each
125 participant’s raw score was obtained by adding the scores of each scale. The raw scores and the ages of the infants were displayed in a graph in order to identify the percentile of infant neurobiological - motor development. In this study, we used the AIMS score for analysis. Three experienced physiotherapists who were blinded for the outcomes applied the AIMS testing. A concordance analysis was performed at the beginning and in the middle of the measurements. Peripheral venous blood samples (5 ml) were collected in anticoagulant-free tubes (vacutainer system) from all subjects between 08:00 and 10:00 p.m. The samples were immediately centrifuged at 5,000 x g for 10 min, and the serum was stored at -80°C until analysis. Serum levels of NGF, BDNF and IL-6 were measured using a commercially available enzyme immunoassay kit (DuoSet ELISA Development, R&D Systems Inc., USA). The amount of BDNF, NGF and IL-6 was determined by measuring the absorbance at 450 nm with a SpectraMax M5 spectrophotometer (Molecular Devices, USA). All samples and standards were measured in duplicate, and the coefficient of variation was less than 5% (Haas et al., 2010). The serum NGF, BDNF and IL-6 levels are expressed as ng/ml. Saliva samples (1 ml) were collected in sterile microtubes from all subjects between 08:00 and 10:00 p.m. The samples were immediately centrifuged at 5,000 x g for 10 min, and the supernatant was stored at -80°C until analysis. Salivary cortisol was measured in duplicate samples with a commercially available High Sensitivity Salivary Cortisol enzyme immunoassay kit (Salimetrics®, USA). The amount of cortisol was determined by measuring the absorbance at 450 nm as described above. All samples and standards were measured in duplicate, and the coefficient of variation was less than 5%. The salivary cortisol levels are expressed as µg/dl. Data was double-entered into an Epi Info 6.04d software application, and an internal consistency check was conducted. Statistical analyses were performed using the Stata
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1 UNIVERSIDADE CATÓLICA DE PELOTAS
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3 Transtornos do humor no período
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5 AGRADECIMENTOS Ao meu orientador
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7 ÍNDICE INTRODUÇÃO GERAL ......
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9 INTRODUÇÃO GERAL Diversas alter
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11 PARTE I - PROJETO I. IDENTIFICA
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13 nos casos em que o suicídio é
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15 IX. Os problemas de sono em beb
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17 oportunizando menos interações
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19 risco de desordens psicopatológ
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21 I V. MÉTODOS 4.1. Delineamento
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23 identificar crianças com atraso
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25 Um questionário com variáveis
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27 4.5. Pessoal Envolvido A equipe
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29 Trinta dias após o nascimento d
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31 4.12. Cronograma Atividades 1 2
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33 12.Glasser, D. Child abuse and n
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35 33.Kapczinski F, Quevedo J. Tran
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VI. ANEXOS 37
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ANEXO 6.2 EDINBURGH POSTNATAL SCALE
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68 ( ) Sim, na maioria das vezes. (
Page 76 and 77: ANEXO 6.4 QUESTIONÁRIO DE CAPTAÇ
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Page 100 and 101: 100 Consentimento Livre e Esclareci
Page 102 and 103: 102 Encaminhamentos ESTUDO SOBRE SA
Page 104 and 105: 104 Conclusions Clinicians should e
Page 106 and 107: 106 investigated whether serum BDNF
Page 108 and 109: 108 characteristics of the sample,
Page 110 and 111: 110 treatment and SLEs in pregnancy
Page 112 and 113: 112 comes from a variety of studies
Page 114 and 115: 114 11. PANDEY GN, REN X, RIZAVI HS
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Page 118 and 119: 118 Figure Captions Figure 1 Serum
Page 120 and 121: 120 Artigo 2: Serum NGF, BDNF and I
Page 122 and 123: 122 INTRODUCTION Several studies ha
Page 126 and 127: 126 Statistical Package, version 9.
Page 128 and 129: 128 A correlation within the biomar
Page 130 and 131: 130 relationship and that when the
Page 132 and 133: 132 not provide an adequate means o
Page 134 and 135: 134 12.Trevarthen C, Aitken KJ. Inf
Page 136 and 137: 136 32.Cirulli F, Francia N, Berry
Page 138 and 139: 138 Table 1 Sample characteristics
Page 140 and 141: 140 Artigo 3: Chronicity and severi
Page 142 and 143: 142 1. Introduction Infant sleep pr
Page 144 and 145: 144 assumed level of significance o
Page 146 and 147: 146 The results of the present stud
Page 148 and 149: 148 Gregory, A. M., e O’Connor, T
Page 150 and 151: 150 PARTE III - CONSIDERAÇÕES FIN
Page 152 and 153: 152 níveis de BDNF podem estar ass
Page 154 and 155: 154 HIPÓTESE X - Os transtornos do
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Karen Amaral Tavares Pinheiro - Universidade Católica de Pelotas

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