Laiza Maria Grassi Fais - Faculdade de Odontologia - Unesp

14Fais LMG. Surface topography and Streptococcus mutans adhesion totitanium after brushing with fluoride and fluoride-free toothpaste simulating10 years of use [Tese de Doutorado]. Araraquara: Faculdade deOdontologia da UNESP; 2011.AbstractTitanium exposure to fluorides could degrade themetal surface, contributing to an increased roughness and providingadditional sites to micro-organisms adhesion. The aim of this study was tocontrasting titanium surface topography after procedures that simulated10 years of brushing using toothpastes with or without fluoride. Ti-6Al-4V(Group L – N=36) and commercially pure titanium (cp Ti – Group T; N=36)disks (6mm Ø X 4mm) were mirror-polished and treated according to 6subgroups (n=6) as a function of immersion (I) or brushing (B) usingdistilled water (W), distilled water + fluoride-free toothpaste (T) anddistilled water + fluoride toothpaste (1,500 ppm; pH = 6.3) (FT).Specimens submitted to immersion were submerged in the vehicleswithout brushing for 244 h ( 10 years). For brushed specimens,procedures were carried out in a linear brushing machine with a softbristledtoothbrush (244 h; 60 cycles/min). Surface topography wasevaluated at baseline (pre-treatment) and post-treatment, using atomicforce microscope in order to obtain three-dimensional (3D) images andmean roughness (Ra). IFT and BFT samples were analyzed underscanning electron microscope with dispersive spectrometer (EDX). Forthe adhesion test, the disks were contaminated with standard strain of S.mutans (NTCC 1023) and the adhesion was analyzed by the colonyformingunit counts (CFU/mL) of the adhered viable cell and by scanningelectron microscopy (SEM) images. The subgroups were compared withone-way ANOVA and Tukey post-test (α=.05). Pre and post-treatmentvalues of Ra were compared using the paired Student T test (α=.05). At